Related Experiment Video

Updated: Jun 8, 2026

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
13:36

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches

Published on: May 6, 2015

Mucosal adenovirus-vectored vaccine for measles

Liubov M Lobanova1, Tayyba T Baig, Suresh K Tikoo

  • 1Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, S7N 5E3 Canada.

Vaccine
|October 5, 2010
PubMed

Insights

A new single-shot measles vaccine (Ad-F/H) delivered intranasally effectively generates measles virus (MV)-specific antibodies and protects against MV replication, even in the presence of pre-existing antibodies.

Area of Science:

  • Virology
  • Vaccinology
  • Immunology

Background:

  • Existing measles vaccines face challenges, necessitating improved single-shot alternatives.
  • A need exists for a measles vaccine effective via mucosal administration and functional despite pre-existing neutralizing antibodies.

Purpose of the Study:

  • To develop a novel single-shot anti-measles vaccine.
  • To evaluate the efficacy of a recombinant adenovirus-vectored vaccine (Ad-F/H) administered via mucosal (intranasal) and intramuscular routes.
  • To assess vaccine functionality in the presence of anti-measles neutralizing antibodies.

Main Methods:

  • Construction of two recombinant human adenoviruses (Ad-F/H) encoding measles virus (MV) fusion (F) and hemagglutinin (H) proteins.
  • Vaccination of mice and cotton rats via intranasal or intramuscular routes.
  • Measurement of MV-specific serum neutralizing antibodies and IgA titers.
  • Challenge studies to assess protection against MV replication in lungs.

Main Results:

  • Intranasal or intramuscular Ad-F/H vaccination induced high MV-specific serum neutralizing-antibody titers in mice and cotton rats.
  • Intranasal vaccination led to a 100-fold increase in MV-specific IgA titers in mice compared to intramuscular vaccination.
  • Intranasal Ad-F/H administration completely protected cotton rats from MV lung replication, while intramuscular administration did not.

Conclusions:

  • The Ad-F/H vaccine is a promising candidate for a single-shot measles vaccine.
  • Intranasal administration elicits robust mucosal immunity (IgA) and superior protection against MV replication.
  • This vaccine strategy shows potential for overcoming challenges associated with current measles vaccines.

More Related Videos

Cationic Nanoemulsion-Encapsulated Retinoic Acid as an Adjuvant to Promote OVA-Specific Systemic and Mucosal Responses06:02

Cationic Nanoemulsion-Encapsulated Retinoic Acid as an Adjuvant to Promote OVA-Specific Systemic and Mucosal Responses

Published on: February 23, 2024

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo12:42

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

Related Concept Videos

Respiratory Syncytial Virus Disease01:29

Respiratory Syncytial Virus Disease

Human respiratory syncytial virus (RSV) is a widespread pathogen that primarily targets infants and young children but also poses a serious health risk to elderly and immunocompromised individuals. Belonging to the Pneumoviridae family, RSV is a negative-sense, single-stranded RNA virus within the Pneumovirus genus. Its global health burden is significant, with millions of cases annually resulting in hospitalizations and mortality, particularly in resource-limited settings. Although most...
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.