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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...

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Related Experiment Video

Updated: Jun 8, 2026

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

Automated data collection for electron microscopic tomography.

Shawn Q Zheng1, J W Sedat, D A Agard

  • 1The Howard Hughes Medical Institute, University of California, San Francisco, California, USA.

Methods in Enzymology
|October 5, 2010
PubMed
Summary
This summary is machine-generated.

UCSF Tomography software automates electron microscopic tomography (EMT) data collection and real-time reconstruction. This enhances efficiency and robustness in 3D volume reconstruction for cryo-electron microscopy.

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Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
11:33

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Published on: January 30, 2016

Related Experiment Videos

Last Updated: Jun 8, 2026

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
11:33

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Published on: January 30, 2016

Area of Science:

  • Structural biology
  • Microscopy techniques
  • Computational imaging

Background:

  • Automated data collection in electron microscopic tomography (EMT) is crucial for efficiency and robustness.
  • Existing methods often lack real-time feedback, potentially limiting throughput.
  • Developing integrated solutions for target finding, data collection, and reconstruction is needed.

Purpose of the Study:

  • To introduce UCSF Tomography, a software solution for automated single and dual-axis EMT.
  • To enable efficient and robust data collection through predictive methods and real-time reconstruction.
  • To facilitate full automation from target identification to 3D volume reconstruction.

Main Methods:

  • Developed a predictive data collection strategy based on geometric rotation assumptions.
  • Integrated real-time reconstruction using weighted back-projection on a parallel cluster.
  • Implemented a hierarchical scheme for target finding and relocation in dual-axis EMT.

Main Results:

  • Achieved dynamic prediction of image movement (15nm in x-y, 100nm in focus) without additional imaging.
  • Demonstrated moderate resolution reconstructions in parallel with data collection.
  • Enabled automated target finding, data collection, and 3D volume reconstruction with minimal user intervention.

Conclusions:

  • UCSF Tomography provides an inclusive, automated solution for electron microscopic tomography.
  • The integrated approach enhances efficiency and robustness in cryo-electron tomography workflows.
  • The software is freely available for nonprofit use, promoting wider adoption.