Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Membrane protein solubilization and structure determination using de novo-designed proteins.

Science (New York, N.Y.)·2026

Same author

CRISPR-based environmental detection of Burkholderia pseudomallei identifies sanitation gaps and melioidosis risk in northeast Thailand.

Nature communications·2026

Same author

Whole-genome sequencing to investigate the prevalence and transmission of multidrug-resistant Gram-negative pathogens in an adult intensive care unit in the UK.

Microbial genomics·2026

Same author

Evolution of pandemic cholera at its global source.

Nature·2026

Same author

Cholera in Kenya: A scoping review of current research, evidence gaps and future directions.

PLOS global public health·2026

Same author

Identification of transporter-dependent capsular loci associated with the invasive potential of Escherichia coli.

Nature microbiology·2026

Same journal

The role of the antimicrobial peptide nisin as a clean label food preservative.

Current opinion in microbiology·2026

Same journal

From coarse-grained metabolic rules to fine-grained control of microbial communities.

Current opinion in microbiology·2026

Same journal

Progress in engineered bacterial cancer therapies.

Current opinion in microbiology·2026

Same journal

Constraints on adaptive loss-of-function mutations during microbial metabolic interactions.

Current opinion in microbiology·2026

Same journal

Discovery of novel antimicrobials within microbiomes.

Current opinion in microbiology·2026

Same journal

Beyond the protein lattice: bacterial S-layer glycans - from structure to functional frontier.

Current opinion in microbiology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 8, 2026

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

Studying bacterial transcriptomes using RNA-seq.

Nicholas J Croucher¹, Nicholas R Thomson

¹The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, Cambridgeshire, CB10 1SA, UK.

Current Opinion in Microbiology

|October 5, 2010

Summary

This summary is machine-generated.

Second-generation sequencing, or RNA-sequencing (RNA-seq), offers superior bacterial gene expression analysis over microarrays. This advanced technique reveals novel RNA sequences and a complex transcriptional landscape, improving our understanding of bacterial genomes.

More Related Videos

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Published on: November 7, 2025

AQRNA-seq for Quantifying Small RNAs

AQRNA-seq for Quantifying Small RNAs

Published on: February 2, 2024

Related Experiment Videos

Last Updated: Jun 8, 2026

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Published on: November 7, 2025

AQRNA-seq for Quantifying Small RNAs

AQRNA-seq for Quantifying Small RNAs

Published on: February 2, 2024

Area of Science:

Microbiology
Genomics
Molecular Biology

Background:

Bacterial gene expression studies are transitioning from microarray technology to next-generation sequencing.
RNA-sequencing (RNA-seq) presents significant advantages over hybridization-based methods.

Purpose of the Study:

To highlight the benefits and emerging applications of RNA-seq in bacterial transcriptomics.
To discuss the impact of RNA-seq on understanding bacterial genome content and RNA biology.

Main Methods:

Utilizing second-generation sequencing platforms for genome-wide transcriptional profiling.
Adapting and refining RNA-seq protocols for specific RNA biology investigations.

Main Results:

RNA-seq enables annotation-independent transcription detection, enhanced sensitivity, and a wider dynamic range.
Studies using RNA-seq have identified numerous novel coding and non-coding RNA sequences.
The bacterial transcriptional landscape revealed by RNA-seq shows increasing similarity to eukaryotic systems.

Conclusions:

RNA-seq is revolutionizing the study of bacterial gene expression and genome content.
Ongoing protocol refinements promise deeper insights into bacterial RNA biology and gene regulation.