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Updated: Jun 8, 2026

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
Published on: February 5, 2020
Single-particle electron microscopy of animal fatty acid synthase describing macromolecular rearrangements that
Edward J Brignole1, Francisco Asturias
1Department of Cell Biology, The Scripps Research Institute, La Jolla, California, USA.
Abstract:
We have used macromolecular electron microscopy (EM) to characterize the conformational flexibility of the animal fatty acid synthase (FAS). Here we describe in detail methods employed for image collection and analysis. We also provide an account of how EM results were interpreted by considering a high-resolution static FAS X-ray structure and functional data to arrive at a molecular understanding of the way in which conformational pliability enables fatty acid synthesis.

