Identification of a red-emitting fluorescent ligand for in vitro visualization of human serotonin 5-HT(1A) receptors
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Updated: Jun 8, 2026
Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
Published on: August 20, 2012
Enza Lacivita1, Anna Carmela Masotti, Md Jafurulla
1Università degli Studi di Bari A. Moro, Dipartimento Farmaco-Chimico, via Orabona, 4, 70125 Bari, Italy.
Abstract:
The 5-HT(1A) receptor subtype is the most thoroughly studied serotonin receptor subtype. We report here the design, synthesis and characterization of two new fluorescent ligands for the 5-HT(1A) receptor. The new 1-arylpiperazine-based red-emitting fluorescent compound 6 displayed good binding affinity at the 5-HT(1A) receptor (K(i)=35 nM) and was able to label specifically the human 5-HT(1A) receptor stably expressed in CHO cells visualized using confocal laser scanning microscopy.
