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Updated: Jun 8, 2026

An Improved Method to Isolate Mitochondrial Contact Sites
Published on: June 16, 2023
Functional annotation of heart enriched mitochondrial genes GBAS and CHCHD10 through guilt by association
Ruben S R M Martherus1, Willem Sluiter, Erika D J Timmer
1Department of Clinical Genomics, Maastricht University, Universiteitssingel 50, P.O. Box 616, 6200 MD, Maastricht, The Netherlands. ruben.martherus@maastrichtuniversity.nl
Abstract:
Despite the mitochondria ubiquitous nature many of their components display divergences in their expression profile across different tissues. Using the bioinformatics-approach of guilt by association (GBA) we exploited these variations to predict the function of two so far poorly annotated genes: Coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) and glioblastoma amplified sequence (GBAS). We predicted both genes to be involved in oxidative phosphorylation. Through in vitro experiments using gene-knockdown we could indeed confirm this and furthermore we asserted CHCHD10 to play a role in complex IV activity.
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