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Related Concept Videos

High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...

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Related Experiment Video

Updated: Jun 8, 2026

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
13:28

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

Published on: May 16, 2017

Single particle analysis at high resolution.

Yao Cong1, Steven J Ludtke

  • 1National Center for Macromolecular Imaging, The Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, USA.

Methods in Enzymology
|October 5, 2010
PubMed
Summary
This summary is machine-generated.

Electron cryomicroscopy (cryo-EM) is advancing 3D biomolecular structure determination. High-resolution cryo-EM methods are now accessible for non-virus particles, enabling near-native structural insights.

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High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

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Related Experiment Videos

Last Updated: Jun 8, 2026

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
13:28

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

Published on: May 16, 2017

Cryo-EM and Single-Particle Analysis with Scipion
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Cryo-EM and Single-Particle Analysis with Scipion

Published on: May 29, 2021

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
08:48

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

Published on: April 28, 2022

Area of Science:

  • Structural Biology
  • Biophysics
  • Biochemistry

Background:

  • Electron cryomicroscopy (cryo-EM) with single particle analysis is a powerful technique for determining the 3D structure of biomolecules.
  • Recent advancements have improved cryo-EM resolution to sub-nanometer and beyond 0.5 nm.

Purpose of the Study:

  • To review and discuss methodologies for high-resolution cryo-EM structure determination of non-virus particles.
  • To focus on reconstruction strategies within the EMAN software suite.

Main Methods:

  • Sub-nanometer resolution is achievable with mid-range microscopes and basic specimen requirements.
  • High-resolution cryo-EM beyond 0.5 nm requires high-end microscopes, careful data handling, and stringent specimen preparation.

Main Results:

  • The chapter details methods for achieving high resolution in cryo-EM for non-virus particles.
  • It specifically highlights the reconstruction strategy used in the EMAN software.

Conclusions:

  • Cryo-EM is a rapidly evolving technique for near-native structural analysis of large biomolecules.
  • The methodologies discussed provide a pathway to obtaining high-resolution structures of non-virus particles.