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Updated: Feb 7, 2026
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Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
Published on: April 25, 2025
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Full-field time-resolved fluorescence tomography of small animals
Vivek Venugopal1, Jin Chen, Frederic Lesage
1Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
Optics Letters
|October 5, 2010
Summary
This study shows wide-field illumination improves time-resolved fluorescence molecular tomography. This technique accurately locates fluorescent markers in small animal models for preclinical applications.
Area of Science:
- Biomedical optics
- Medical imaging
- Fluorescence tomography
Background:
- Time-resolved fluorescence molecular tomography (TR-FMT) is an advanced imaging technique.
- Wide-field illumination offers potential advantages for TR-FMT systems.
- Evaluating wide-field illumination's performance is crucial for preclinical applications.
Purpose of the Study:
- To investigate the feasibility of wide-field illumination for TR-FMT.
- To assess the performance of wide-field patterns in both in vitro and in vivo settings.
- To compare time-gated data with continuous-wave (cw) data for improved image reconstruction.
Main Methods:
- Utilized a time-resolved imaging platform with wide-field illumination patterns.
- Employed a Monte Carlo-based forward model for fluorescence yield reconstruction.
- Conducted experiments in vitro and with a small animal model (murine).
Main Results:
- Demonstrated improved resolution and quantification using time-gated data compared to cw data in vitro.
- Successfully achieved accurate localization of a fluorescent inclusion in a murine chest cavity.
- Established the feasibility of wide-field strategies for preclinical fluorescence imaging.
Conclusions:
- Wide-field illumination is a viable approach for time-resolved fluorescence molecular tomography.
- Time-gated data significantly enhances image quality in TR-FMT.
- This method shows promise for accurate preclinical fluorescence imaging applications.
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