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A rapid method for the purification of RNA polymerase holoenzyme from Escherichia coli
1Department of Biochemistry, College of Medicine, East Tennessee State University, Johnson City 37614.
Journal of Biochemical and Biophysical Methods
|November 1, 1990
Abstract:
A method is described for the rapid purification of RNA polymerase holoenzyme from small amounts of Escherichia coli cells. Chromatography of a crude extract on a single-stranded DNA agarose column followed by gel filtration chromatography gave 95% pure holoenzyme. The enzyme had kinetic characteristics on T7 DNA identical to those of RNA polymerase purified by other more laborious procedures.