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Polymer-protein conjugates. I. Effect of protein conjugation on the cloud point of poly (N-isopropylacrylamide)
J P Chen1, H J Yang, A S Hoffman
1Center for Bioengineering, FL-20, University of Washington, Seattle 98195.
Biomaterials
|November 1, 1990
Abstract:
Poly (N-isopropylacrylamide) (p(NIPAAm] exhibits a cloud point around 32 degrees C. In this study we have conjugated several proteins to p(NIPAAm), and the effects of protein molecular weight, pH and ionic strength on the cloud point of the p(NIPAAm)-protein conjugates have been studied. The cloud points of p(NIPAAm)-protein conjugates appear to be unaffected by the molecular weight or isoelectric point (pl) of the proteins, the pH or ionic strength.