Related Experiment Video
Updated: Aug 4, 2026
Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies
Published on: February 16, 2010
Isolation of a vegetalizing inducing factor after extraction with acid ethanol. Concentration-dependent inducing
1Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, F.R.G.
Abstract:
A vegetalizing factor has been isolated from chicken embryos by an improved method. The factor is extracted with acid/ethanol and finally purified by four consecutive steps of reversed phase HPLC. The molecular mass is about 25 kDa. The factor dissociates after reduction with dithiothreitol into two subunits of about 13 kDa. The factor was tested on Triturus alpestris by the implantation method, and on isolated ectoderm of Xenopus laevis in solution. The factor induces as the crude fractions all types of mesodermal tissues dependent on the concentration of the factor.
More Related Videos
09:57Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
Published on: June 14, 2020
06:53Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023