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Computer simulation for optimization of high-performance liquid chromatography of some phenolic pollutants
W Markowski1, T H Dzido, E Soczewiński
1Department of Inorganic and Analytical Chemistry, Medical Academy, Lublin, Poland.
Abstract:
Computer simulation was used to optimize high-performance liquid chromatography of phenol and its chloro and nitro derivatives. On the basis of two linear gradient runs of different steepness (RP-18-water + methanol + 1% acetic acid), several simulated gradient runs allowed the optimum gradient programme and flow-rate to be chosen so that the time of analysis could be considerably shortened. Good agreement between simulated and experimental chromatograms was obtained in spite of changes in experimental conditions.
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