Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Separation of lipid classes by solid phase extraction.

H Y Kim1, N Salem

  • 1Section of Analytical Chemistry, LCS, DICBR, NIAAA, Bethesda, MD 20892.

Journal of Lipid Research
|December 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Probing lepton flavour violation via neutrinoless [Formula: see text] decays with the ATLAS detector.

The European physical journal. C, Particles and fields·2017
Same author

Search for new phenomena in events with at least three photons collected in <i>pp</i> collisions at [Formula: see text] = 8 TeV with the ATLAS detector.

The European physical journal. C, Particles and fields·2017
Same author

A measurement of the calorimeter response to single hadrons and determination of the jet energy scale uncertainty using LHC Run-1 <i>pp</i>-collision data with the ATLAS detector.

The European physical journal. C, Particles and fields·2017
Same author

Measurement of the [Formula: see text] and [Formula: see text] production cross sections in multilepton final states using 3.2 fb[Formula: see text] of [Formula: see text] collisions at [Formula: see text] = 13 TeV with the ATLAS detector.

The European physical journal. C, Particles and fields·2017
Same author

A facile synthesis of 3,4-dimercaptofurans via sulfenylation of (E)-β-chlorovinyl ketones and 1,2-sulfur migration.

Organic & biomolecular chemistry·2017
Same author

Endpoints for screening thyroid cancer in the Republic of Korea: thyroid specialists' perspectives.

Journal of endocrinological investigation·2016
Same journal

The Metabolic-Immune Axis in MASH: Is the Macrophage Signature the Key to Liver Disease Staging?

Journal of lipid research·2026
Same journal

BK channel deficiency promotes lipolysis via an AKT-independent activation of the cAMP/PKA/HSL pathway.

Journal of lipid research·2026
Same journal

Interleukin-6 stimulates platelet 12-lipoxygenase to drive coagulation in inflammatory arthritis.

Journal of lipid research·2026
Same journal

LIPA regulates myeloid differentiation and is essential for intra-plaque macrophage accumulation during atherogenesis.

Journal of lipid research·2026
Same journal

Hydrolysis and depletion of phosphatidylglycerol at peak murine acute lung injury.

Journal of lipid research·2026
Same journal

Multispecies transcriptome analysis identifies networks protecting against MASLD in Göttingen minipigs.

Journal of lipid research·2026
See all related articles

This study presents a fast, reliable method using aminopropyl columns to separate neutral and acidic phospholipid fractions. The technique ensures high recovery of various lipid classes without degradation, simplifying lipid analysis.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Lipidomics

Background:

  • Accurate separation of lipid classes is crucial for understanding cellular processes.
  • Existing methods may have limitations in recovery or degradation of certain lipid species.

Purpose of the Study:

  • To develop a rapid and reliable method for separating diverse lipid classes.
  • To improve the recovery of acidic phospholipids and prevent molecular species degradation.

Main Methods:

  • Utilized aminopropyl disposable columns for lipid separation.
  • Modified an existing procedure with specific solvent systems for elution.
  • Analyzed recovery and molecular integrity of various lipid classes including cholesterol, triglycerides, and phospholipids.

Related Experiment Videos

Main Results:

  • Achieved high recoveries for cholesterol, triglycerides, free fatty acids, and multiple phospholipid classes (PC, PE, PI, PS, PA, SM).
  • Demonstrated no preferential loss or degradation of specific molecular species, confirmed by fatty acid distribution and molecular profiles.
  • The method effectively separates lipid classes based on polarity, yielding pure or mixed fractions.

Conclusions:

  • The described method offers a rapid, reliable, and high-recovery approach for lipid class separation.
  • This technique simplifies subsequent analyses like thin-layer chromatography or high-performance liquid chromatography.
  • It is a valuable tool for comprehensive lipidomic studies across various biological samples.