Preparative scale isolation of sphingosine
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1Mental Health Research Institute, University of Michigan, Ann Arbor 48109.
Journal of Lipid Research
|December 1, 1990
Abstract:
A high-yield method is described for the preparation of sphingenine from galactosylceramide. Two successive cleavage reactions are used, alkaline butanol to form galactosylsphingosine, then acidic acetonitrile to form the sphingenine. A column purification procedure utilizing silica gel is described. The method is superior to previously described methods in its rapidity and avoidance of isomerization and contamination by racemization and rearrangement products.