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Isolation, Fixation, and Immunofluorescence Imaging of Mouse Adrenal Glands
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How useful is immunofluorescence in tropical renal pathology?

A Date1, M Pant

  • 1Department of Pathology, Christian Medical College Hospital, Vellore, Tamil Nadu, India.

Transactions of the Royal Society of Tropical Medicine and Hygiene
|July 1, 1990
PubMed

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Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...