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Related Experiment Videos

raf oncogenes in carcinogenesis.

S M Storm1, U Brennscheidt, G Sithanandam

  • 1Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick, MD 21701-1013.

Critical Reviews in Oncogenesis
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

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The raf gene family, including c-raf, A-raf, and B-raf, encodes protein kinases involved in cell signaling. Aberrant raf gene activation, through mutations or truncations, can contribute to cancer development.

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • The raf gene family comprises key regulators of intracellular signaling pathways.
  • Understanding raf gene structure and function is crucial for deciphering oncogenesis.

Purpose of the Study:

  • To characterize the structure, expression, and activation mechanisms of mammalian raf genes.
  • To explore the role of raf genes in carcinogenesis.

Main Methods:

  • Comparative analysis of raf gene structures across species.
  • Gene mapping and RNA expression profiling.
  • Biochemical characterization of raf protein kinases.

Main Results:

  • Identified three active raf genes in humans (c-raf, A-raf, B-raf) with distinct genomic structures and expression patterns.

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  • Defined conserved regions (CR1, CR2, CR3) within raf proteins, including the kinase domain (CR3).
  • Demonstrated that deletions or point mutations can activate raf genes, with truncated versions like v-raf implicated in oncogenesis.

    • Conclusions:

    • Raf proteins are cytoplasmic serine/threonine kinases with conserved functional domains.
    • Aberrant activation of c-raf-1, particularly through truncation or mutation, is a potential mechanism for oncogene activation in cancer.