Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
Colloids03:22

Colloids

Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles that are visible to the naked eye or can be seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. On the other hand, a solution is a homogeneous mixture in which no settling occurs and in which the dissolved...
Micelles01:30

Micelles

Micelle formation is an intricate process that hinges on the properties of amphiphilic or amphipathic molecules and the conditions of the system in which they are found. Amphiphilic molecules, which have both hydrophilic (water-attracting) and hydrophobic (water-repelling) parts, play a critical role in this process.In aqueous environments, these molecules arrange themselves such that their hydrophilic heads are turned towards the water phase, while their hydrophobic tails are oriented away...
Surface Active Agents01:27

Surface Active Agents

Surfactants, named for their behavior at interfaces, positively adsorb at the interfaces of two phases, reducing interfacial tension. Their versatility as emulsifiers, detergents, and foaming agents stems from this ability. Surfactants, often termed amphiphiles, share the property of amphipathy, with molecules having both hydrophilic and hydrophobic portions. The hydrophilic part is called the head, and the hydrophobic part, including an elongated alkyl substituent, forms the tail.Surfactants...
Lipid-Lowering Drugs: Statins and Miscellaneous Agents01:20

Lipid-Lowering Drugs: Statins and Miscellaneous Agents

Hyperlipidemia, a medical condition often referred to as high cholesterol, is characterized by abnormally elevated levels of lipids in the bloodstream. When present in excess, these lipids, specifically cholesterol and triglycerides, can lead to serious health complications, often involving cardiovascular diseases. Illnesses like atherosclerosis, heart attacks, and pancreatitis have all been linked to untreated hyperlipidemia. This means controlling and regulating cholesterol and triglyceride...
Solubility03:00

Solubility

Solution, Solubility, and Solubility Equilibrium
A solution is a homogeneous mixture composed of a solvent, the major component, and a solute, the minor component. The physical state of a solution—solid, liquid, or gas—is typically the same as that of the solvent. Solute concentrations are often described with qualitative terms such as dilute (of relatively low concentration) and concentrated (of relatively high concentration).
In a solution, the solute particles (molecules, atoms, and/or ions)...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Work-school conflict and turnover intention among Chinese Music University students with part-time jobs: the roles of negative affect and resilience.

Frontiers in psychology·2026
Same author

Association of Diabetes Mellitus and Malnutrition with <i>Aspergillus</i> IgG and Chronic Pulmonary Aspergillosis in Pulmonary Tuberculosis Patients: A Retrospective Study.

Infection and drug resistance·2026
Same author

Protein-enhanced small molecule disruptors of ordered membrane domains.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Stable and Tunable Expression of Human Peripheral Myelin Protein 22 in Rat Schwann Cells.

The Journal of biological chemistry·2026
Same author

Ribosomal protein L8 promotes melanoma progression by regulating the cell cycle and metastasis.

Acta biochimica et biophysica Sinica·2026
Same author

<i>ZNF25</i> as an immunotherapy target: pan-cancer biomarker potential and mechanistic exploration in glioma.

Frontiers in oncology·2026
Same journal

Aromatic Cage-Directed Azide-Methyllysine Photochemistry for Profiling Nonhistone Interacting Partners of the MeCP2 Methyl-CpG-Binding Domain.

Biochemistry·2026
Same journal

Differential Hydroxypyruvate Processing by <i>E. coli</i> and <i>P. aeruginosa</i> DXP Synthases Reveals Preferential Xylulose 5-Phosphate Formation by the <i>P. aeruginosa</i> Enzyme.

Biochemistry·2026
Same journal

Structural and Functional Characterization of Heterologous Nitrogenase Complexes.

Biochemistry·2026
Same journal

Discovery of Bacterial Unspecific Peroxygenases.

Biochemistry·2026
Same journal

Lactate Biology: Subcellular Routing and Chemical Form Define Function.

Biochemistry·2026
Same journal

Nature's Anaerobic Toolkit: Glycyl Radical Enzymes and Their Expanding Functional and Mechanistic Diversity.

Biochemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 8, 2026

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
10:12

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Published on: March 25, 2020

CHOBIMALT: a cholesterol-based detergent.

Stanley C Howell1, Ritesh Mittal, Lijun Huang

  • 1Department of Biochemistry, Center for Structural Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.

Biochemistry
|October 6, 2010
PubMed
Summary
This summary is machine-generated.

A new cholesterol-based detergent, CHOBIMALT, forms stable micelles for membrane protein studies. When added to existing detergents, it significantly enhances the thermal stability of solubilized G-protein coupled receptors.

More Related Videos

ABCG5/G8 Crystallization in a Lipidic Bicelle Environment for X-Ray Crystallography
06:47

ABCG5/G8 Crystallization in a Lipidic Bicelle Environment for X-Ray Crystallography

Published on: August 25, 2023

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
10:27

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Published on: October 21, 2018

Related Experiment Videos

Last Updated: Jun 8, 2026

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
10:12

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Published on: March 25, 2020

ABCG5/G8 Crystallization in a Lipidic Bicelle Environment for X-Ray Crystallography
06:47

ABCG5/G8 Crystallization in a Lipidic Bicelle Environment for X-Ray Crystallography

Published on: August 25, 2023

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
10:27

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Published on: October 21, 2018

Area of Science:

  • Biochemistry
  • Membrane Protein Solubilization
  • Detergent Chemistry

Background:

  • Cholesterol derivatives are crucial for membrane protein studies but exhibit poor aqueous solubility.
  • Existing cholesterol derivatives often form viscous solutions or fail to create conventional micelles.

Purpose of the Study:

  • To synthesize and characterize a novel cholesterol-based detergent, CHOBIMALT.
  • To evaluate CHOBIMALT's micelle-forming properties and its ability to solubilize membrane proteins.
  • To assess the impact of CHOBIMALT on the thermal stability of solubilized membrane proteins.

Main Methods:

  • Synthesis and characterization of CHOBIMALT.
  • Micelle formation assessment using diffusion and fluorescence measurements.
  • Evaluation of CHOBIMALT's efficacy in solubilizing the human kappa opioid receptor type 1 (hKOR1).

Main Results:

  • CHOBIMALT self-assembles into large micelles (210±30 kDa) above 3–4 µM without requiring heat or sonication.
  • CHOBIMALT alone was not an effective surfactant for membrane extraction.
  • In combination with classical detergents, CHOBIMALT significantly improved the thermal stability of solubilized hKOR1.

Conclusions:

  • CHOBIMALT is a novel cholesterol-based detergent capable of forming stable micelles.
  • While not a standalone surfactant, CHOBIMALT enhances the thermal stability of membrane proteins when used with conventional detergents.
  • CHOBIMALT shows promise for improving membrane protein structural and functional studies.