Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Oxygen Requirements and Growth Patterns

Oxygen Requirements and Growth Patterns

Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...

Electron Transport Chain: Complex III and IV

Electron Transport Chain: Complex III and IV

During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...

Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide

Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide

Alkenes are converted to 1,2-diols or glycols through a process called dihydroxylation. It involves the addition of two hydroxyl groups across the double bond with two different stereochemical approaches, namely anti and syn. Dihydroxylation using osmium tetroxide progresses with syn stereochemistry.

Preparation and Reactions of Thiols

Preparation and Reactions of Thiols

Thiols are prepared using the hydrosulfide anion as a nucleophile in a nucleophilic substitution reaction with alkyl halides. For instance, bromobutane reacts with sodium hydrosulfide to give butanethiol.

Catalytically Perfect Enzymes

Catalytically Perfect Enzymes

The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.

The Supercomplexes in the Crista Membrane

The Supercomplexes in the Crista Membrane

The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Characterisation of an oxygen-tolerant recombinant nitroreductase for development of an aryl amine biosynthesis platform using cofactor-regenerating crude extract system.

Journal of hazardous materials·2026

Same author

Interaction of environmental fluoride exposure and gut microbes: Potential implication in the development of fluorosis in human subjects.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2025

Same author

Health risk modeling and risk factors of fluorosis in the fluoride endemic village of Maharashtra: A cross-sectional study.

Environmental monitoring and assessment·2024

Same author

Estimated exposure to microplastics through national and local brands of bottled water in Central India.

Environmental monitoring and assessment·2024

Same author

Strategy for Remediation of Polychlorinated Biphenyls-Contaminated Soil Through Redox Management Based on Electronegativity of the Contaminants.

Bulletin of environmental contamination and toxicology·2023

Same author

Potential of Antarctic lipase from Acinetobacter johnsonii Ant12 for treatment of lipid-rich wastewater: screening, production, properties and applications.

Biodegradation·2023

Search research articles

Related Experiment Video

Updated: Jun 8, 2026

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Published on: February 24, 2018

Superoxide dismutase: an industrial perspective.

Amit Bafana¹, Som Dutt, Sanjay Kumar

¹Biotechnology Division, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, Palampur, Himachal Pradesh, India. abafana@rediffmail.com

Critical Reviews in Biotechnology

|October 6, 2010

Summary

Superoxide dismutase (SOD) shows industrial promise but faced limitations in therapeutic use. This review examines SOD

More Related Videos

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Published on: May 4, 2018

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Published on: January 11, 2019

Related Experiment Videos

Last Updated: Jun 8, 2026

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Published on: February 24, 2018

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Published on: May 4, 2018

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Published on: January 11, 2019

Area of Science:

Biotechnology and Industrial Enzyme Applications
Biochemistry and Enzyme Engineering
Antioxidant Enzyme Research

Background:

Enzyme technologies are crucial in industrial R&D and manufacturing.
Biotechnology advancements enable safer, potent, and specific enzyme production.
Antioxidant enzymes, particularly superoxide dismutase (SOD), are gaining industrial significance.

Purpose of the Study:

To review the industrial trajectory of superoxide dismutase (SOD).
To analyze the reasons behind SOD's limited therapeutic success.
To identify future research directions and industrial applications for SOD.

Main Methods:

Literature review of industrial enzyme applications and SOD research.
Analysis of SOD's historical market performance and limitations.
Exploration of enzyme engineering, production, and optimization strategies for SOD.

Main Results:

SOD initially showed therapeutic promise but faced performance issues.
Its industrial use shifted to non-drug human applications and animal drug applications.
Significant research is ongoing, indicating continued industrial interest.

Conclusions:

The industrial application of SOD requires addressing past limitations.
Further research in enzyme engineering and production optimization is essential.
SOD holds potential for specialized industrial and therapeutic applications with further development.