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Related Concept Videos

2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...

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Related Experiment Video

Updated: Jun 8, 2026

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

Characteristics of zero-quantum correlation spectroscopy in MAS NMR experiments.

Stephanie G Köneke1, Jacco D van Beek, Matthias Ernst

  • 1ETH Zürich, Physical Chemistry, Wolfgang-Pauli-Strasse 10, 8093 Zürich, Switzerland.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|October 6, 2010
PubMed
Summary

This study optimizes zero-quantum coherence generation in solid-state NMR using novel pulse sequences. These advancements improve efficiency and spectral resolution for analyzing complex molecules like ubiquitin.

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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

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Last Updated: Jun 8, 2026

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Area of Science:

  • Solid-state Nuclear Magnetic Resonance (NMR) spectroscopy
  • Quantum coherence phenomena

Background:

  • Magic-angle spinning (MAS) solid-state NMR is crucial for studying molecular structure and dynamics.
  • Zero-quantum coherence (ZQC) generation and reconversion are key techniques for enhancing spectral resolution and simplifying complex spectra.
  • Efficient ZQC generation is often limited by factors such as proton decoupling and pulse sequence design.

Purpose of the Study:

  • To analyze and optimize zero-quantum coherence generation and reconversion in magic-angle spinning solid-state NMR.
  • To develop and validate efficient symmetry-based pulse sequences for ZQC measurements.
  • To explore the application of ZQC techniques for improved spectral resolution in solid samples.

Main Methods:

  • Implementation of symmetry-based pulse sequences utilizing isotropic J couplings or dipolar couplings for ZQC generation.
  • Crucial role of abundant proton spin decoupling for efficient ZQC generation.
  • Development of optimized sequences for zero-quantum single-quantum (ZQ-SQ) correlation spectra measurement.
  • Investigation of ZQ-SQ correlation spectroscopy in ubiquitin and polypeptides.

Main Results:

  • Achieved 50% efficiency in ZQ-SQ correlation spectra measurements in ubiquitin.
  • Demonstrated high efficiency and selectivity in ZQ-SQ experiments on polypeptides.
  • Identified increased line widths in the multiple-quantum (MQ) dimension as a trade-off.
  • Compared ZQ-SQ spectroscopy with single-quantum single-quantum (SQ-SQ) and double-quantum single-quantum (DQ-SQ) correlation spectroscopy.

Conclusions:

  • Optimized ZQC generation and reconversion methods enhance solid-state NMR experiments.
  • ZQ-SQ correlation spectroscopy offers advantages in spectral resolution for certain applications.
  • Proton decoupling is critical for efficient ZQC generation in MAS NMR.
  • Further investigation into ZQ-SQ applications in polypeptides shows promise for improved spectral analysis.