Related Experiment Video
Updated: Jun 8, 2026
05:32
In Vivo Osteo-organoid Approach for Harvesting Therapeutic Hematopoietic Stem/Progenitor Cells
Published on: February 16, 2024
Osteoplant acts on stem cells derived from peripheral blood
Vincenzo Sollazzo1, Annalisa Palmieri, Ambra Girardi
1Orthopedic Clinic, Corso Giovecca 203, 44100 Ferrara, Italy.
Journal of Indian Society of Periodontology
|October 6, 2010
Summary
Osteoplant, a bone graft material, promotes osteoblast differentiation in stem cells by upregulating key bone-related genes. This study clarifies its mechanism for bone regeneration.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Orthopedic Research
Background:
- Osteoplant is a reabsorbable equine bone graft used for skeletal repair.
- Its precise mechanism in promoting bone formation via osteoblast activity is not fully understood.
Purpose of the Study:
- To investigate how Osteoplant influences osteoblast differentiation in mesenchymal stem cells.
- To elucidate the molecular mechanisms underlying Osteoplant-induced bone regeneration.
Main Methods:
- Mesenchymal stem cells were treated with Osteoplant.
- Gene expression analysis of bone-related markers and stem cell markers was performed using real-time Reverse Transcription-Polymerase Chain Reaction (RT-PCR).
Main Results:
- Osteoplant significantly induced osteoblast transcriptional factors (RUNX2) and bone-related genes (SPP1, BGLAP).
- A significant decrease in the mesenchymal stem cell marker ENG (CD105) was observed, confirming Osteoplant's differentiation effect.
Conclusions:
- Osteoplant effectively promotes osteoblast differentiation in mesenchymal stem cells.
- These findings enhance understanding of bone regeneration mechanisms and provide a model for evaluating similar biomaterials.
Related Concept Videos
01:21Stem Cell Therapy for Tissue Regeneration
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell types that...
Types of Stem Cells used in Stem Cell Therapy
The two main cell types that...
01:01Regulation of Hematopoietic Stem Cells
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
01:19Multipotency of Hematopoietic Stem Cells
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
01:19Mesenchymal Stem Cells
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...
01:17Stem Cell Culture
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
01:34Production of Formed Elements
Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...
Most HSCs commit to...