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Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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Related Experiment Video

Updated: Jun 8, 2026

Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food
08:38

Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food

Published on: May 6, 2013

Listeria monocytogenes: knowledge gained through DNA sequence-based subtyping, implications, and future

Kendra Nightingale1

  • 1Colorado State University, Department of Animal Sciences, Fort Collins, CO 80523, USA. kendra.nightingale@colostate.edu

Journal of AOAC International
|October 7, 2010
PubMed
Summary

Bacterial subtyping differentiates isolates beyond species. Molecular methods, particularly DNA sequencing, offer advanced discrimination for pathogens like Listeria monocytogenes, improving food safety analysis.

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Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
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Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

Published on: August 9, 2011

Related Experiment Videos

Last Updated: Jun 8, 2026

Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food
08:38

Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food

Published on: May 6, 2013

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
12:50

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

Published on: August 9, 2011

Area of Science:

  • Microbiology
  • Genomics
  • Food Safety

Background:

  • Bacterial subtyping is crucial for differentiating isolates beyond species level.
  • Methods include phenotypic (metabolite expression) and molecular (nucleic acid interrogation) approaches.
  • Listeria monocytogenes is a key foodborne pathogen requiring precise subtyping.

Purpose of the Study:

  • To review bacterial subtyping concepts.
  • To discuss molecular subtyping methods for Listeria monocytogenes.
  • To emphasize DNA sequence-based subtyping advancements.

Main Methods:

  • Overview of phenotypic and molecular subtyping categories.
  • Focus on band-based vs. DNA sequence data generation methods.
  • Analysis of Listeria monocytogenes genetic lineages.

Main Results:

  • Molecular subtyping classifies Listeria monocytogenes into four genetic lineages.
  • Band-based methods remain standard for routine subtyping.
  • Increasing DNA sequence data drives development of new high-throughput methods.

Conclusions:

  • DNA sequence-based subtyping offers high discrimination for Listeria monocytogenes.
  • Advancements in sequencing technology enhance pathogen characterization.
  • Improved subtyping aids in tracking and controlling foodborne pathogens.