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Updated: Jun 8, 2026

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
12:11

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

Published on: October 23, 2009

Multi-residue screening method to quantify mycotoxins in aqueous environmental samples.

Judith Schenzel1, René P Schwarzenbach, Thomas D Bucheli

  • 1Agroscope Reckenholz-Tänikon Research Station ART, CH-8046 Zurich, Switzerland.

Journal of Agricultural and Food Chemistry
|October 8, 2010
PubMed
Summary

This study introduces a new method to detect over 30 mycotoxins in water. Beauvericin and nivalenol were found in river and drainage water, highlighting environmental risks.

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Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Ecotoxicology

Background:

  • Mycotoxins, fungal metabolites, contaminate agricultural products.
  • Previous research confirmed zearalenone and deoxynivalenol in aquatic environments.
  • The presence of other mycotoxins in natural waters requires investigation.

Purpose of the Study:

  • To develop and validate a method for analyzing approximately 30 additional mycotoxins in various water types.
  • To quantify specific mycotoxins in drainage, river, and wastewater effluent.
  • To assess the environmental occurrence of mycotoxins beyond previously studied compounds.

Main Methods:

  • Solid-phase extraction using Oasis HLB cartridges.
  • Liquid chromatography coupled with electrospray ionization triple quadrupole mass spectrometry (LC-ESI-MS/MS).
  • Method validation including recovery and detection limits.

Main Results:

  • The validated method achieved >70% recovery for 13 out of 33 mycotoxins in wastewater effluent.
  • 27 compounds had method detection limits below 10 ng/L.
  • Beauvericin and nivalenol were quantified in drainage and river water for the first time.

Conclusions:

  • A robust analytical method for a wider range of mycotoxins in water was established.
  • Beauvericin and nivalenol are present in drainage and river systems.
  • Further ecotoxicological risk assessment of these mycotoxins in natural waters is necessary.