Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acoustic Steering Using Thermally Induced Optical Reflection of Sound (THORS).

Applied spectroscopy·2021
Same author

Controlling Microarray Feature Spreading and Response Stability on Porous Silicon Platforms by Using Alkene-Terminal Ionic Liquids and UV Hydrosilylation.

Langmuir : the ACS journal of surfaces and colloids·2020
Same author

Demonstration of a Human Color Vision Mimic in the Infrared.

Analytical chemistry·2019
Same author

Interplay Between Silicon Nanocrystal Size and Local Environment Within Porous Silicon on the Analyte-Dependent Photoluminescence Response.

Applied spectroscopy·2019
Same author

Effects of Acetone Vapor on the Exciton Band Photoluminescence Emission from Single- and Few-Layer WS<sub>2</sub> on Template-Stripped Gold.

Sensors (Basel, Switzerland)·2019
Same author

Infrared Reflectance Spectroscopic Evaluation of Inkjet Printed Standards of Cyclotrimethylenetrinitramine (RDX) on Aluminum Substrates.

Applied spectroscopy·2018

Related Experiment Video

Updated: Jun 8, 2026

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements
05:49

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Published on: December 2, 2022

Dynamics within site selectively templated and tagged xerogel sensor platforms.

Frank V Bright1, Ellen L Holthoff

  • 1Department of Chemistry, Natural Sciences Complex, University at Buffalo, The State University of New York, Buffalo, New York 14260-3000, USA. chefvb@buffalo.edu

Applied Spectroscopy
|October 8, 2010
PubMed
Summary

This study introduces a novel sensor platform using nitrobenzo-2-oxa-1,3-diazole (NBD) reporter molecules. Increased n-octyl content enhances analyte binding and sensor performance by altering local microviscosity.

More Related Videos

Microfabricated Platforms for Mechanically Dynamic Cell Culture
15:21

Microfabricated Platforms for Mechanically Dynamic Cell Culture

Published on: December 26, 2010

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

Related Experiment Videos

Last Updated: Jun 8, 2026

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements
05:49

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Published on: December 2, 2022

Microfabricated Platforms for Mechanically Dynamic Cell Culture
15:21

Microfabricated Platforms for Mechanically Dynamic Cell Culture

Published on: December 26, 2010

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

Area of Science:

  • Chemical Sensors
  • Materials Science
  • Fluorescence Spectroscopy

Background:

  • Nitrobenzo-2-oxa-1,3-diazole (NBD) based sensors utilize reporter molecules for signal generation.
  • Site selectively templated and tagged xerogel (SSTTX) platforms offer tunable microenvironments.
  • Understanding the interplay between molecular structure and sensor response is crucial for analytical applications.

Purpose of the Study:

  • To investigate the role of local microviscosity and molecular crowding in an NBD-based SSTTX sensor.
  • To evaluate the impact of n-octyl residue content on sensor performance and analyte binding.
  • To elucidate the mechanism of analyte-dependent fluorescence signaling.

Main Methods:

  • Fabrication of NBD-based SSTTX sensor platforms with varying n-octyl content.
  • Fluorescence spectroscopy to monitor NBD reporter molecule signals.
  • Analysis of local microviscosity using fluorescence lifetime measurements.
  • Correlation of microviscosity and molecular crowding with analyte binding affinity.

Main Results:

  • The SSTTX platform exhibits distinct responsive (Site 1) and non-responsive (Site 2) NBD reporter sites.
  • Analyte binding to Site 1 decreases photo-induced electron transfer (PET) efficiency, modulating fluorescence.
  • Local microviscosity around Site 1 NBD molecules significantly increases upon analyte binding (from ~260 cP to ~540 cP without n-octyl).
  • Increasing n-octyl content further elevates local microviscosity (to ~360 cP and ~760 cP) and enhances analyte binding.

Conclusions:

  • The n-octyl chains induce molecular crowding around the responsive NBD sites, enhancing sensor performance.
  • Local microviscosity is a critical factor influencing the photo-induced electron transfer (PET) efficiency and analyte detection.
  • The SSTTX platform demonstrates tunable properties for improved analytical applications through controlled molecular architecture.