GPCRRD: G protein-coupled receptor spatial restraint database for 3D structure modeling and function annotation.
1Center for Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Ave, Ann Arbor, MI 48109, USA.
Bioinformatics (Oxford, England)
|October 8, 2010
Summary
The GPCRRD database collects experimental data on G protein-coupled receptors (GPCRs), crucial drug targets. This resource aids in modeling GPCR structures and understanding their functions.
Area of Science:
- Biochemistry
- Structural Biology
- Pharmacology
Background:
- G protein-coupled receptors (GPCRs) are the largest family of integral membrane proteins.
- GPCRs represent a critical class of drug targets in medicine.
- Limited crystal structures exist for GPCRs, necessitating alternative methods for structural insights.
Purpose of the Study:
- To create the GPCRRD database for systematic collection of experimental restraints for GPCRs.
- To aid in the 3D structure modeling and functional annotation of GPCRs.
- To consolidate data from literature and primary resources using automated text mining and manual validation.
Main Methods:
- Automated text mining algorithms to extract experimental restraints from literature.
- Manual validation of extracted data for accuracy and reliability.
- Systematic collection of residue orientation, contact, and distance maps.
Main Results:
- The GPCRRD database now contains thousands of spatial restraints.
- Data is sourced from diverse experimental techniques including mutagenesis, disulfide mapping, cryo-EM, and FTIR spectroscopy.
- The database provides a comprehensive resource for GPCR structural studies.
Conclusions:
- The GPCRRD database is a valuable tool for GPCR 3D structure modeling.
- It facilitates functional annotation of GPCRs by providing experimental evidence.
- The database supports research on GPCRs as drug targets.
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