Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pilot- and process-scale techniques for cell disruption.

H Schütte1, M R Kula

  • 1GBF-Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Federal Republic of Germany.

Biotechnology and Applied Biochemistry
|December 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

New method for analysing spatial relationships of facial muscles on MRI: a pilot study.

International journal of oral and maxillofacial surgery·2024
Same author

Isotopic Control of the Boron-Vacancy Spin Defect in Hexagonal Boron Nitride.

Physical review letters·2023
Same author

The average three-dimensional face for different sex and age groups in a Dutch population.

International journal of oral and maxillofacial surgery·2023
Same author

Locking in trochanteric fractures: a comparison of static versus dynamic locking using the Gamma3 nail.

European journal of orthopaedic surgery & traumatology : orthopedie traumatologie·2022
Same author

[The unresolved contest between MTA and IRM as apical barrier material in apicoectomies. A retrospective cohort study].

Nederlands tijdschrift voor tandheelkunde·2022
Same author

Microfluidic approach for controlled ultraviolet treatment of colored and fluorescent dissolved organic matter.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2020
Same journal

Development and Validation of an ELISA Method for Quantification of HuB01 in Fermentation Products.

Biotechnology and applied biochemistry·2026
Same journal

Optimization and Characterization of Poly-γ-Glutamic Acid Production From a Locally Isolated Bacillus cereus ARM24.

Biotechnology and applied biochemistry·2026
Same journal

Isolation and Characterization of a Novel Microbacterium takaoensis Lipase for Nutritional and Textural Improvement of Pizza Dough.

Biotechnology and applied biochemistry·2026
Same journal

Targeting Amastigote and Trypomastigote Phases: Multi-Epitope Vaccine Strategy Against Trypanosoma cruzi.

Biotechnology and applied biochemistry·2026
Same journal

Novel Inhibitors Targeting Class B1 Metallo-β-Lactamases in Carbapenem-Resistant Acinetobacter baumannii.

Biotechnology and applied biochemistry·2026
Same journal

Formulation of PLA/Chitosan Copolymeric Nanocarriers Enhanced the Sustained and Targeted Drug Delivery of Cinnamic Acid: In Vitro Drug Optimization and In Vivo Biodistribution in Rat Model.

Biotechnology and applied biochemistry·2026
See all related articles

Releasing intracellular proteins from microorganisms requires cell disruption. Mechanical methods are versatile for industrial protein isolation, while chemical or enzymatic approaches offer selectivity for specific applications.

Area of Science:

  • Biotechnology
  • Industrial Microbiology
  • Protein Chemistry

Background:

  • Microorganisms are valuable sources of proteins with significant industrial and therapeutic potential.
  • Isolation of intracellular proteins necessitates effective cell disruption techniques.
  • Optimizing protein recovery is crucial for commercial viability.

Purpose of the Study:

  • To review and compare different cell disruption methods for protein isolation from microorganisms.
  • To emphasize scale-up considerations and industrial applicability of various techniques.
  • To guide process design by evaluating yield, cost, and downstream implications.

Main Methods:

  • Review of chemical, biological, and physical cell disruption approaches.
  • Emphasis on mechanical cell disintegration devices and their improvements.

Related Experiment Videos

  • Analysis of chemical extraction and enzymatic lysis for protein release.

    • Main Results:

    • Mechanical cell disruption methods are generally well-suited for large-scale industrial applications.
    • Chemical and enzymatic methods provide higher selectivity but require tailored procedures.
    • Process design must balance disruption efficiency with downstream processing, particularly solid/liquid separation.

    Conclusions:

    • The choice of cell disruption method depends on the specific protein product and desired scale.
    • Mechanical disruption offers broad applicability, while enzymatic and chemical methods allow for targeted isolation.
    • Integrated process design considering yield, cost, and subsequent recovery steps is essential for successful industrial protein production.