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[Effect of dureping injection on T-cells function and their function in killing FM1 infected Mphi in vitro]
Shan Jing1, Li-gang Gu, Yun Zhou
1Key Laboratory of Antivirus of the Ministry of Education, Beijing University of Chinese Medicine, Beijing.
Objective:
To investigate the effect of Dureping Injection (DRP) on the T-cells function of mice and the function of T-cells in killing MF infected by influenza virus subtype A mice-lung adaptive strain FM1 in vitro.
Methods:
Number of splenic normal and FM1 infected T-cells in mice were measured by MTT and double-antibody sandwich ELISA, after being treated with DRP at different concentrations (2.1, 8.5 and 17.0 microg/mL), and the effect of DRP on interferon-gamma (IFN-gamma) and interleukin-10 (IL-10) production as well as on splenic T-cell killing FM1 infected Mphi/Ana-1 function were detected.
Results:
DRP inhibited the multiplication of normal spleen T cells induced by concanavalin A in vitro, suppressed Th2 cell factor IL-10 production, and maintained Th1 cell factor IFN-y at a definite level, moreover, it directly enhanced the power of T-cells in killing FM1 infected Mphi (P < 0.05, P < 0.01).
Conclusion:
DRP could act on mice T-cells to enhance the immune response for antiinfluenza viral FM1 in vitro.
Insights
Dureping Injection (DRP) enhances T-cell immune response against influenza virus FM1 in mice. It boosts T-cell killing of infected cells while modulating cytokine production for better anti-viral immunity.
Area of Science:
- Immunology
- Virology
- Pharmacology
Context:
- Influenza virus subtype A (FM1) poses a significant threat to respiratory health.
- T-cells play a crucial role in orchestrating adaptive immune responses against viral infections.
- Understanding modulators of T-cell function is key to developing novel antiviral strategies.
Purpose:
- To evaluate the impact of Dureping Injection (DRP) on murine T-cell function.
- To assess DRP's effect on T-cell-mediated killing of influenza virus-infected cells in vitro.
- To investigate DRP's influence on key cytokine production (IFN-γ and IL-10).
Summary:
- DRP treatment modulated T-cell responses in vitro.
- It inhibited normal T-cell proliferation induced by concanavalin A.
- DRP suppressed Interleukin-10 (IL-10) production while maintaining Interferon-gamma (IFN-γ) levels, and significantly enhanced T-cell-mediated killing of FM1-infected macrophages.
Impact:
- DRP demonstrates potential as an immunomodulatory agent for enhancing anti-influenza immunity.
- The findings suggest DRP could bolster T-cell responses against influenza virus FM1.
- Further research may explore DRP's therapeutic applications in managing influenza infections.
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