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Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Imaging Biological Samples with Optical Microscopy01:18

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
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Published on: April 11, 2013

A new wave of cellular imaging.

Derek Toomre1, Joerg Bewersdorf

  • 1Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520-8002, USA. derek.toomre@yale.edu

Annual Review of Cell and Developmental Biology
|October 9, 2010
PubMed
Summary

Super-resolution nanoscopy techniques break the diffraction limit for detailed molecular imaging. These advanced fluorescence microscopy methods reveal cellular processes with unprecedented resolution and sensitivity.

Area of Science:

  • Optics and Photonics
  • Cell Biology
  • Biophysics

Background:

  • Conventional fluorescence microscopy is limited by the diffraction limit (~200 nm).
  • Super-resolution nanoscopy techniques have emerged to overcome this limitation.
  • These methods offer significantly enhanced resolution for biological imaging.

Purpose of the Study:

  • To review the principles, design, and applications of super-resolution nanoscopy.
  • To highlight advances and common themes in these powerful imaging techniques.
  • To discuss the impact of nanoscopy on cell biology research.

Main Methods:

  • Examination of optical principles behind techniques like STED, (F)PALM/STORM, and structured illumination.
  • Analysis of instrument design for achieving high resolution and sensitivity.

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  • Discussion of probe state conversion and scanning strategies.

    • Main Results:

    • Super-resolution nanoscopy achieves tens of nanometers resolution, enabling single-molecule visualization.
    • Techniques offer unique strengths in resolution, speed, sensitivity, and fluorophore compatibility.
    • Nanoscopes have revealed transient states in living cells, leading to new discoveries.

    Conclusions:

    • Super-resolution nanoscopy represents a major advance in biological imaging.
    • These techniques provide unprecedented insights into molecular and cellular dynamics.
    • Future developments promise even greater capabilities for biological discovery.