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Related Concept Videos

Production of Pharmaceuticals01:30

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...

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Surface Engineering of Pancreatic Islets with a Heparinized StarPEG Nanocoating
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Published on: June 23, 2018

Polyelectrolyte multilayer films: A sponge for insulin?

Nadia Ladhari1, Joseph Hemmerlé, Youssef Haikel

  • 1Université de Strasbourg, Faculté de Chirurgie Dentaire, Strasbourg, France. Nadia.Ladhari@odonto-ulp.u-strasbg.fr

Bio-Medical Materials and Engineering
|October 9, 2010
PubMed
Summary
This summary is machine-generated.

Researchers developed polyelectrolyte multilayer (PEM) films to support insulin reservoirs for progressive hormone delivery. These films show controlled insulin release in saline solutions, offering a potential new method for diabetes treatment.

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Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • Polymer Chemistry

Background:

  • Diabetes management requires advanced insulin administration strategies.
  • Current treatments face challenges with restrictive delivery methods.
  • Polyelectrolyte multilayer (PEM) films offer potential for controlled release applications.

Purpose of the Study:

  • To develop a novel PEM film-based support for insulin reservoirs.
  • To investigate the loading and progressive release of insulin from these films.
  • To assess the feasibility of PEM films for new insulin delivery systems.

Main Methods:

  • Insulin loading into (PDADMAC-PAA)n films via immersion.
  • Confocal laser scanning microscopy (CLSM) for visualizing insulin distribution.
  • Environmental scanning electron microscopy (ESEM) for film morphology.
  • Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy for release studies.

Main Results:

  • Insulin diffused into PEM films, with concentration varying by immersion time and pH.
  • Film swelling was observed during insulin loading.
  • No significant insulin release occurred in distilled water.
  • Slow insulin release was detected in a 0.15 M NaCl solution, mimicking physiological conditions.

Conclusions:

  • PEM films can be loaded with insulin and exhibit controlled release behavior.
  • The release rate is dependent on the surrounding solution's salinity.
  • This approach presents a promising avenue for developing innovative insulin delivery devices.