Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...
Neurulation01:30

Neurulation

Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the anterior...
Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
Gastrulation01:56

Gastrulation

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata will form...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Osr1-expressing mesoderm contributes to lymphatic vessel assembly and complexity in the mammalian kidney.

Cell reports·2026
Same author

CreER activation transiently disrupts angiogenesis by reducing proliferation and promoting apoptosis in vascular endothelial cells.

Angiogenesis·2026
Same author

Neuropilin 1 (NRP1) conveys SEMA3A signals to restrict physiological angiogenesis.

Angiogenesis·2026
Same author

Exploiting porphyrin metabolism to inhibit angiogenesis.

Angiogenesis·2026
Same author

Neural crest cell-derived DKK1 and NEDD4 modulate Wnt signalling in the second heart field to orchestrate outflow tract development.

Nature communications·2026
Same author

Discovery of New Markers for Haemogenic Endothelium and Haematopoietic Progenitors in the Mouse Yolk Sac.

Journal of developmental biology·2026

Related Experiment Video

Updated: Jun 8, 2026

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
12:48

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

Published on: June 2, 2012

In the beginning: Generating neural crest cell diversity.

Christiana Ruhrberg1, Quenten Schwarz

  • 1Institute of Opthalmology, University College London.

Cell Adhesion & Migration
|October 9, 2010
PubMed
Summary
This summary is machine-generated.

Neural crest cells (NCCs) development involves understanding when their fate is determined. This review explores prespecification, where NCCs may already possess a determined fate at birth, influencing their diverse cell type generation.

More Related Videos

Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation
09:03

Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation

Published on: February 7, 2012

Cranial Neural Crest Cells Three-Dimensional In Vitro Differentiation Protocol for Multiplexed Assay
08:55

Cranial Neural Crest Cells Three-Dimensional In Vitro Differentiation Protocol for Multiplexed Assay

Published on: February 14, 2025

Related Experiment Videos

Last Updated: Jun 8, 2026

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
12:48

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

Published on: June 2, 2012

Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation
09:03

Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation

Published on: February 7, 2012

Cranial Neural Crest Cells Three-Dimensional In Vitro Differentiation Protocol for Multiplexed Assay
08:55

Cranial Neural Crest Cells Three-Dimensional In Vitro Differentiation Protocol for Multiplexed Assay

Published on: February 14, 2025

Area of Science:

  • Developmental biology
  • Cell biology
  • Genetics

Background:

  • Neural crest cells (NCCs) are crucial migratory cells originating from the neural tube during embryonic development.
  • NCCs differentiate into a wide array of cell types, forming key components of the vertebrate body plan.
  • The precise timing of NCC fate determination remains a central question in developmental biology.

Purpose of the Study:

  • To review recent advancements in understanding cell fate specification in trunk NCCs.
  • To explore the role of prespecification versus environmental signaling in NCC development.
  • To synthesize current knowledge on how NCC diversity is established.

Main Methods:

  • This review synthesizes findings from various experimental studies on NCC development.
  • It analyzes genetic and molecular data related to NCC fate determination.
  • The focus is on trunk NCCs and their developmental trajectories.

Main Results:

  • Evidence suggests that NCCs may possess a degree of prespecification regarding their future lineage at the time of their delamination from the neural tube.
  • Environmental cues from target tissues likely interact with this prespecified potential, guiding differentiation.
  • The interplay between intrinsic potential and extrinsic signals contributes to the remarkable diversity of NCC derivatives.

Conclusions:

  • Prespecification plays a significant role in trunk neural crest cell development, challenging purely environment-driven fate determination models.
  • Understanding prespecification offers insights into how complex cell types arise during embryogenesis.
  • Further research into the molecular mechanisms of prespecification will illuminate NCC developmental pathways.