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Failure of ciprofloxacin to eradicate brucellosis in experimental animals
I O al-Orainey1, A M Bashandi, E N Saeed
1Department of Medicine, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
Abstract:
Ciprofloxacin is a quinolone that penetrates well intracellularly. It was shown to be active against Brucella melitensis in vitro. In this study, ciprofloxacin and tetracycline were used to treat B. melitensis and B. abortus infections in mice. The drugs were given for 7, 14, 21 and 28 days. Tetracycline reduced spleen and liver weight and splenic bacterial counts and eliminated the infection in 70% of mice treated for 3 or 4 weeks. Ciprofloxacin, on the other hand, did not affect liver or spleen weight or bacterial counts after 3-4 weeks of therapy. It failed to eradicate the organism from any of the mice treated. It is concluded that ciprofloxacin is probably not suitable for the treatment of brucellosis.
Insights
Ciprofloxacin is not effective for treating brucellosis in mice. Tetracycline successfully cleared Brucella infections, while ciprofloxacin failed to eradicate the bacteria, indicating it
Area of Science:
- Infectious Diseases
- Microbiology
- Pharmacology
Background:
- Brucellosis is a zoonotic disease caused by Brucella species.
- Intracellular penetration of antibiotics is crucial for treating brucellosis.
- Ciprofloxacin, a quinolone, exhibits intracellular activity and in vitro activity against Brucella melitensis.
Purpose of the Study:
- To evaluate the efficacy of ciprofloxacin compared to tetracycline in treating experimental Brucella melitensis and Brucella abortus infections in mice.
Main Methods:
- Mice infected with B. melitensis and B. abortus were treated with ciprofloxacin or tetracycline.
- Treatment durations included 7, 14, 21, and 28 days.
- Efficacy was assessed by monitoring spleen and liver weights, splenic bacterial counts, and infection eradication.
Main Results:
- Tetracycline treatment reduced organ weights and bacterial loads, eradicating infection in 70% of mice after 3-4 weeks.
- Ciprofloxacin did not significantly alter spleen or liver weights or bacterial counts.
- Ciprofloxacin failed to eradicate Brucella infection in any treated mice.
Conclusions:
- Ciprofloxacin is likely unsuitable for treating brucellosis due to its ineffectiveness in this murine model.
- Tetracycline demonstrated significant efficacy in clearing Brucella infections in mice.
- Further research may be needed to identify optimal antibiotic regimens for brucellosis.