Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Transgenic Organisms00:53

Transgenic Organisms

Overview
Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NUP107-160 nuclear pore sub-complex members determine symbiotic ion channel localization in legumes.

Plant physiology·2026
Same author

MtGRF8 interacts with MtSymCRK and prevents early bacteroid death during Medicago - Sinorhizobium symbiosis.

The New phytologist·2026
Same author

Understanding Rice Responses to the Combination of Bacterial Panicle Blight and High Night Temperatures.

Rice (New York, N.Y.)·2026
Same author

An artificial neural network-based deep learning model to predict combined stress impact and interaction in plants.

Applications in plant sciences·2026
Same author

The F-Box Protein DWARF1 Controls Plant Height by Modulating MtSMXL6 and MtSMXL7 Stability in Medicago truncatula.

Plant, cell & environment·2026
Same author

The TERMINAL FLOWER1-NODULE ROOTs-FRUITFULLc module orchestrates compound inflorescence development in Medicago truncatula.

The Plant cell·2026

Related Experiment Video

Updated: Jun 8, 2026

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
07:33

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Published on: January 3, 2014

Agroinoculation and agroinfiltration: simple tools for complex gene function analyses.

Zarir Vaghchhipawala1, Clemencia M Rojas, Muthappa Senthil-Kumar

  • 1Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, OK, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 9, 2010
PubMed
Summary
This summary is machine-generated.

Agroinoculation and agroinfiltration are versatile plant gene analysis tools. These methods facilitate functional gene analysis, genetic screens, and studies of plant-pathogen interactions and gene expression.

More Related Videos

Vacuum-Forced Agroinfiltration for In planta Transformation of Recalcitrant Plants: Cacao as a Case Study
05:52

Vacuum-Forced Agroinfiltration for In planta Transformation of Recalcitrant Plants: Cacao as a Case Study

Published on: November 17, 2023

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
07:50

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

Published on: July 23, 2013

Related Experiment Videos

Last Updated: Jun 8, 2026

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
07:33

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Published on: January 3, 2014

Vacuum-Forced Agroinfiltration for In planta Transformation of Recalcitrant Plants: Cacao as a Case Study
05:52

Vacuum-Forced Agroinfiltration for In planta Transformation of Recalcitrant Plants: Cacao as a Case Study

Published on: November 17, 2023

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
07:50

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

Published on: July 23, 2013

Area of Science:

  • Plant Science
  • Molecular Biology
  • Genetics

Background:

  • Agroinoculation, initially for plant-virus interactions, evolved into agroinfiltration for broader plant gene function studies.
  • Advancements in genomics and vector technology have expanded the utility of these viral gene delivery methods.
  • Agroinfiltration enables transient gene expression analysis for diverse biological investigations.

Purpose of the Study:

  • To provide an overview of agroinoculation and agroinfiltration applications in plant science.
  • To detail laboratory protocols for agroinoculation and agroinfiltration in functional gene analysis.
  • To highlight the use of these techniques in virus-induced gene silencing (VIGS) for genetic screens.

Main Methods:

  • Literature review of agroinoculation and agroinfiltration applications.
  • Description of established agroinoculation protocols for viral gene delivery.
  • Demonstration of agroinfiltration techniques for transient gene expression assays.

Main Results:

  • Agroinoculation and agroinfiltration are effective for functional gene analysis.
  • These methods support diverse applications including protein localization and interaction studies.
  • Virus-induced gene silencing (VIGS) combined with agroinfiltration facilitates genetic screens.

Conclusions:

  • Agroinoculation and agroinfiltration are indispensable tools for plant functional genomics.
  • The described methods offer efficient approaches for gene analysis and genetic screening.
  • These techniques are crucial for advancing research in plant-pathogen interactions and abiotic stress responses.