Related Experiment Video

Updated: Jun 8, 2026

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
12:23

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Published on: May 16, 2017

A novel secreted metzincin metalloproteinase from Bacillus intermedius

Albina R Sabirova1, Natalya L Rudakova, Nelly P Balaban

  • 1Department of Microbiology, Kazan (Volga region) Federal University, Kazan, Russia.

FEBS Letters
|October 12, 2010
PubMed

Insights

Researchers identified a novel metalloproteinase, MprBi, from Bacillus intermedius. This bacterial enzyme shares unique features with eukaryotic metalloproteinases, suggesting a new class of prokaryotic enzymes.

Area of Science:

  • Microbiology
  • Biochemistry
  • Enzymology

Background:

  • Bacillus intermedius 3-19 produces a novel secreted metalloproteinase.
  • Metalloproteinases are crucial enzymes involved in various biological processes.

Purpose of the Study:

  • To identify and characterize the novel metalloproteinase (MprBi) from Bacillus intermedius.
  • To elucidate the structural and functional properties of MprBi.

Main Methods:

  • Gene identification and expression in Bacillus subtilis.
  • Protein purification to homogeneity.
  • Biochemical characterization including mass spectroscopy and Edman degradation.

Main Results:

  • The 19 kDa MprBi protein was successfully purified and sequenced.
  • MprBi possesses a unique active site motif (HEYGHNFGLPHD) and Met-turn, characteristic of the metzincin clan.
  • Sequence analysis revealed similarities to eukaryotic adamalysins.

Conclusions:

  • MprBi represents a novel metzincin metalloproteinase from prokaryotes.
  • MprBi and related Bacillus proteins form a new group of bacterial metalloproteinases.
  • This discovery expands the known diversity of metalloproteinases.

More Related Videos

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

Related Concept Videos

Inhalation Anthrax01:25

Inhalation Anthrax

Anthrax is a zoonotic disease caused by Bacillus anthracis, a Gram-positive, spore-forming bacterium. It primarily affects herbivorous animals but can be transmitted to humans through skin contact, ingestion, or inhalation of spores.Cutaneous anthrax, the most common form, typically results from direct contact with bacterial spores through skin abrasions and is generally less severe. Gastrointestinal anthrax results from eating undercooked or contaminated meat. It affects the mouth, throat, or...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Cytoskeletal Proteins in Bacteria01:29

Cytoskeletal Proteins in Bacteria

Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
Bacterial Toxins01:12

Bacterial Toxins

Bacterial toxins are sophisticated virulence factors that enable pathogenic bacteria to interact with, invade, and damage host tissues. These toxins fall broadly into two types: protein exotoxins, which are secreted into the environment and target specific host receptors, and lipopolysaccharide endotoxins, which are structural components of the bacterial outer membrane released primarily during bacterial lysis or membrane shedding. Exotoxins generally act more selectively, binding to cell...
Production of Pharmaceuticals01:30

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...