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Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
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Updated: Jun 8, 2026

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Published on: July 3, 2020

Modeling Sjögren's syndrome with Id3 conditional knockout mice.

Zengli Guo¹, Hongmei Li, Min Han

¹Institute of Developmental Biology and Molecular Medicine, School of Life Sciences, Fudan University, Shanghai 200433, China.

Immunology Letters

|October 12, 2010

Summary

The Id3 gene is crucial for T cell development. Disrupting Id3 in developing T cells caused autoimmune disease, similar to Sjögren

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Area of Science:

Immunology
Molecular Biology
Genetics

Background:

The Id3 gene plays a critical role in the development and function of B and T cells.
Id3-deficient mice exhibit autoimmune disease resembling human Sjögren's syndrome, with both B and T lymphocytes contributing to the phenotype.

Purpose of the Study:

To investigate the role of Id3 in T cell development and its contribution to autoimmune disease.
To establish a new model for studying the relationship between T cell development and autoimmune conditions.

Main Methods:

Generation of an Id3 conditional knockout mouse model using an LckCre transgene to induce Id3 deletion in developing T cells.
Analysis of thymocyte development and maturation in conditional knockout mice.
Assessment of disease development, including exocrinopathy and lymphocyte infiltration, in the generated mouse model.

Main Results:

Efficient disruption of the Id3 gene in early thymocyte development before T cell receptor (TCR)-mediated positive selection.
Impaired thymocyte maturation in Id3 conditional knockout mice.
Development of exocrinopathy and significant lymphocyte infiltration into salivary glands, mirroring Id3 germline knockout mice.

Conclusions:

Acquired mutations in developing T cells can potentially induce autoimmune disease.
This study provides a novel experimental model for dissecting the intricate links between T cell development and autoimmune pathogenesis.