FCRL6 receptor: expression and associated proteins
Sergey V Kulemzin1, Alina Y Zamoshnikova, Mariya Y Yurchenko
1Institute of Chemical Biology and Fundamental Medicine, Lavrentiev Ave. 8, Novosibirsk, Novosibirsk, Russia.
Researchers developed a new antibody to study the FCRL6 receptor, finding it expressed on CD8 T and NK cells. FCRL6 gene expression changes in autoimmune diseases and HIV, suggesting a role in immune regulation.
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Background:
- Fc receptor-like 6 (FCRL6) is a recently identified receptor within the FCRL family.
- Understanding FCRL6's role is crucial for insights into immune cell function and disease modulation.
Purpose of the Study:
- To generate specific monoclonal antibodies (mAbs) against the FCRL6 receptor.
- To characterize the expression pattern and function of FCRL6 in immune cells.
- To investigate the association of FCRL6 with intracellular signaling molecules.
Main Methods:
- Generation of mouse mAbs against baculovirus-derived recombinant FCRL6 protein.
- Characterization of antibody specificity using Western blotting, Flow Cytometry (FACS), and immunohistochemistry.
- Analysis of FCRL6 gene expression in peripheral blood leukocytes and T cells from healthy individuals and patients with autoimmune diseases, blood disorders, and HIV infection.
- Investigation of FCRL6's interaction with signaling molecules like phosphatases and adaptor proteins.
Main Results:
- The mAb clone 7B2 specifically recognizes a 63kDa FCRL6 protein on CD8 T and CD56 NK cells.
- FCRL6 is expressed in antigen-experienced T cells and its gene expression is abrogated upon mitogenic stimulation.
- FCRL6 gene expression is significantly decreased in peripheral T cells of patients with autoimmune and blood diseases.
- Upregulation of FCRL6 gene expression is observed in late-stage HIV infection.
- FCRL6 recruits SHP-1, SHP-2, SHIP-1, SHIP-2 phosphatases, and Grb2 adaptor protein via its phosphorylated cytoplasmic tyrosines.
Conclusions:
- FCRL6 is a cell surface receptor preferentially expressed on CD8 T and CD56 NK cells.
- FCRL6 expression is dynamically regulated in response to cellular activation and in various disease states, including autoimmune disorders, blood diseases, and HIV infection.
- FCRL6's ability to recruit key signaling phosphatases and adaptor proteins suggests an inhibitory role in immune responses, potentially modulating cytotoxic T lymphocyte (CTL) effector functions in immune disorders.
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