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Updated: Jun 8, 2026

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement
Published on: September 6, 2011
A method of printing uniform protein lines by using flat PDMS stamps
Wei Zhang1, Chang-Ying Xue, Kun-Lin Yang
1Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576, Singapore.
Abstract:
In this paper, we report a method of printing uniform protein lines on glass slides by using UV-treated flat PDMS stamps. Unlike traditional microcontact printing (μCP) which requires microstructured PDMS stamps, this μCP method only requires a flat PDMS stamp, an UV lamp and a number of straight needles. Our results show that lines of bovine serum albumin (BSA), immunoglobin (IgG), anti-biotin, anti-human IgG and anti-mouse IgG can be printed evenly on glass slides by using this μCP method. We also demonstrate that the printed protein lines are suitable for applications such as microfluidic immunoassays.

