Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Transgenic Organisms00:53

Transgenic Organisms

Overview
What is Genetic Engineering?00:49

What is Genetic Engineering?

Overview
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epigenetically mismatched parental centromeres trigger genome elimination in hybrids.

Science advances·2021
Same author

Point Mutations in Centromeric Histone Induce Post-zygotic Incompatibility and Uniparental Inheritance.

PLoS genetics·2015
Same author

Naturally occurring differences in CENH3 affect chromosome segregation in zygotic mitosis of hybrids.

PLoS genetics·2015
Same author

A haploid genetics toolbox for Arabidopsis thaliana.

Nature communications·2014
Same author

Hybrid recreation by reverse breeding in Arabidopsis thaliana.

Nature protocols·2014
Same author

Comparative analysis of tandem repeats from hundreds of species reveals unique insights into centromere evolution.

Genome biology·2013

Related Experiment Video

Updated: Jun 8, 2026

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
09:45

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

Chromosome engineering: power tools for plant genetics.

Simon W L Chan1

  • 1Department of Plant Biology, University of California, Davis, 1 Shields Avenue, Davis, CA 95616, USA. srchan@ucdavis.edu

Trends in Biotechnology
|October 12, 2010
PubMed
Summary

Chromosome engineering advances rapidly enhance plant breeding efficiency. Innovations like instant haploid production and converting meiosis to mitosis offer new ways to accelerate crop improvement and preserve hybrid traits.

More Related Videos

Breeding by Design for Functional Rice with Genome Editing Technologies
09:43

Breeding by Design for Functional Rice with Genome Editing Technologies

Published on: January 3, 2025

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
12:59

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Published on: April 30, 2016

Related Experiment Videos

Last Updated: Jun 8, 2026

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
09:45

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

Breeding by Design for Functional Rice with Genome Editing Technologies
09:43

Breeding by Design for Functional Rice with Genome Editing Technologies

Published on: January 3, 2025

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
12:59

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Published on: April 30, 2016

Area of Science:

  • Plant genetics and breeding
  • Molecular biology
  • Reproductive biology

Background:

  • Chromosome engineering involves manipulating genetic inheritance for improved traits.
  • Plant breeding aims to enhance crop efficiency and resilience.
  • Innovations in genetic technologies are crucial for agricultural advancement.

Purpose of the Study:

  • To review recent chromosome engineering innovations.
  • To highlight their potential to increase plant breeding efficiency.
  • To discuss applications in creating homozygous lines, reverse breeding, and apomixis.

Main Methods:

  • Review of current literature on chromosome engineering techniques.
  • Analysis of methods altering kinetochore protein CENH3 for haploid production.
  • Examination of techniques converting meiosis to mitosis.
  • Discussion of advancements in homologous recombination.

Main Results:

  • Haploid Arabidopsis thaliana produced by altering CENH3, enabling instant homozygous lines.
  • Reverse breeding facilitated by haploid production, ensuring intact parental chromosomes.
  • Meiosis-to-mitosis conversion yields diploid gametes, a step towards apomixis.
  • New homologous recombination methods enhance chromosome engineering applications.

Conclusions:

  • Chromosome engineering offers transformative potential for plant breeding.
  • These technologies can accelerate the development of improved crop varieties.
  • Future applications include preserving hybrid vigor and enabling asexual reproduction via seeds.