Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Chemiluminescence: a sensitive and versatile method for analyte detection.

R S Givens¹, D A Jencen, C M Riley

¹Center for Bioanalytical Research, University of Kansas, Lawrence 66045.

Journal of Pharmaceutical and Biomedical Analysis

|January 1, 1990

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Gastrointestinal: Endoscopic mucosal resection for diagnosis of infiltrating gastric cancer: A case report.

Journal of gastroenterology and hepatology·2016

Same author

A method to predict the equilibrium solubility of drugs in solid polymers near room temperature using thermal analysis.

Journal of pharmaceutical sciences·2012

Same author

Intralesional steroid injection to prevent stricture after endoscopic submucosal dissection for esophageal cancer: a controlled prospective study.

Endoscopy·2012

Same author

Pretreatment of leukemic cells with low-dose decitabine markedly enhances the cytotoxicity of gemtuzumab ozogamicin.

Leukemia·2012

Same author

Phase I clinical study to select a novel oral formulation for ibandronate containing the excipient sodium N-[8-(2-hydroxybenzoyl) amino] caprylate (SNAC).

Die Pharmazie·2012

Same author

Clinical features and outcomes of delayed perforation after endoscopic submucosal dissection for early gastric cancer.

Endoscopy·2010

Same journal

Analyzing the impact of ionizable lipid identity, purity, and stability on lipid nanoparticle performance.

Journal of pharmaceutical and biomedical analysis·2026

Same journal

Application of two-dimensional liquid chromatography as a complementary technique to circular dichroism spectroscopy and high-resolution mass spectrometry for the characterization of GalNAc-siRNA conjugates.

Journal of pharmaceutical and biomedical analysis·2026

Same journal

The transfer of per- and polyfluoroalkyl substances (PFAS) from mother to child: Comparison between maternal and cord blood in an Italian cohort.

Journal of pharmaceutical and biomedical analysis·2026

Same journal

UHPLC/Q-TOF-MS-based blood-component profiling and multi-omics analysis reveal potential protective mechanisms of Shenzhuo Formula against diabetic kidney disease.

Journal of pharmaceutical and biomedical analysis·2026

Same journal

Multi-center study of the recombinant cascade reagent (kinetic chromogenic assay) as an alternative method for bacterial endotoxin testing: Method validation, product suitability, and consistency evaluation with limulus amebocyte lysate.

Journal of pharmaceutical and biomedical analysis·2026

Same journal

Simultaneous enantioselective separation of 2-, 3- and 4-chloromethcathinones using supercritical fluid chromatography-tandem mass spectrometry and its application to human oral fluid samples.

Journal of pharmaceutical and biomedical analysis·2026

See all related articles

Researchers improved a chemiluminescence detection method for luminescent materials by developing a kinetic model for the oxalate ester-hydrogen peroxide reaction, enhancing sensitivity and selectivity.

Area of Science:

Analytical Chemistry
Biochemistry

Background:

Chemiluminescence detection using oxalate ester-hydrogen peroxide oxidation is known for luminescent material detection.
Current methods suffer from low efficiency (<0.1%) and limited selectivity.

Purpose of the Study:

To improve the efficiency and selectivity of oxalate ester-hydrogen peroxide chemiluminescence.
To develop a kinetic model for optimizing light production.

Main Methods:

Investigated the effects of catalysts, reagents, and reaction conditions on light production.
Developed a kinetic model for the oxalate ester-hydrogen peroxide reaction.
Applied the model to liquid chromatography using a "time-dependent emission window" concept.

Main Results:

Related Experiment Videos

The developed kinetic model predicts conditions for maximum light production.
The "time-dependent emission window" concept enhances sensitivity for selected analytes.
Successfully applied the methodology to detect and quantify labeled met- and leu-enkephalins.

Conclusions:

The enhanced chemiluminescence method offers improved sensitivity and selectivity for analyte detection.
The kinetic model provides a predictable framework for optimizing chemiluminescence assays.
This methodology has potential for various bioanalytical applications.