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Updated: Jun 8, 2026

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures
Published on: September 28, 2013
Membrane rafts in plant cells
Sébastien Mongrand1, Thomas Stanislas, Emmanuelle M F Bayer
1Laboratoire de Biogenèse Membranaire, Unité Mixte de Recherche 5200 (UMR 5200) Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux, 146 rue Léo Saignat, 33076 Bordeaux, France.
Abstract:
Over the past five years, the structure, composition and possible functions of membrane raft-like domains on plant plasma membranes (PM) have been described. Proteomic analyses have indicated that a high proportion of proteins associated with detergent-insoluble membranes (DIMs), supposed to contain raft-like domains isolated from the PM, might be involved in signalling pathways. Recently, the dynamic association of specific proteins with the DIM fraction upon environmental stress has been reported. Innovative imaging methods have shown that lateral segregation of lipids and proteins exists at the nanoscale level in the plant PM, correlating detergent insolubility and membrane-domain localization of presumptive raft proteins. These data suggest a role for plant rafts as signal transduction platforms, similar to those documented for mammalian cells.
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