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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Related Experiment Video

Updated: Jun 8, 2026

gP2S, an Information Management System for CryoEM Experiments

gP2S, an Information Management System for CryoEM Experiments

Published on: June 10, 2021

EMDataBank.org: unified data resource for CryoEM.

Catherine L Lawson¹, Matthew L Baker, Christoph Best

¹Department of Chemistry and Chemical Biology and Research Collaboratory for Structural Bioinformatics, Rutgers, The State University of New Jersey, 610 Taylor Road Piscataway, NJ 08854, USA. cathy.lawson@rutgers.edu

Nucleic Acids Research

|October 12, 2010

Summary

This summary is machine-generated.

Cryo-electron microscopy (cryoEM) enables macromolecular structure determination. EMDataBank.org unifies access to cryoEM maps and models from EMDB and PDB, facilitating scientific research.

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Last Updated: Jun 8, 2026

gP2S, an Information Management System for CryoEM Experiments

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Published on: June 10, 2021

Single Particle Cryo-Electron Microscopy: From Sample to Structure

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Area of Science:

Structural Biology
Biophysics
Biochemistry

Background:

Cryo-electron microscopy (cryoEM) is a powerful technique for determining the structures of macromolecules and their complexes.
Access to structural data is crucial for advancing biological research.

Purpose of the Study:

To introduce EMDataBank.org as a unified resource for cryoEM data.
To facilitate the deposition and retrieval of cryoEM maps, models, and metadata.

Main Methods:

Integration of data from the EM Data Bank (EMDB) and the Protein Data Bank (PDB).
Development of a global 'one-stop shop' resource for cryoEM structural data.

Main Results:

EMDataBank.org provides unified public access to cryoEM structural data.
The resource consolidates information from major archives, including EMDB and PDB.

Conclusions:

EMDataBank.org enhances accessibility and usability of cryoEM structural data.
This resource supports the wider scientific community in macromolecular structure research.