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Related Concept Videos

IR Spectrometers01:25

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

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Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
11:55

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Published on: May 28, 2021

Modular infrared microspectrometer with cryosampling capabilities.

F Romero-Borja, D L Schmidt, L T Wood

    Applied Optics
    |October 12, 2010
    PubMed
    Summary
    This summary is machine-generated.

    A new modular infrared spectrometer was developed for analyzing superconducting materials. This instrument features microsampling and cryosampling, offering enhanced optical characterization capabilities.

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    Area of Science:

    • Spectroscopy
    • Materials Science
    • Physics

    Background:

    • Infrared spectroscopy is crucial for material analysis.
    • Characterizing superconducting materials requires specialized instrumentation.
    • Existing methods may have limitations in sampling or temperature control.

    Purpose of the Study:

    • To design and construct a versatile infrared spectrometer.
    • To incorporate microsampling and cryosampling capabilities.
    • To evaluate the instrument's performance for superconducting materials.

    Main Methods:

    • Development of a modular infrared spectrometer.
    • Integration of microsampling accessories.
    • Implementation of a cryosampling stage.
    • Testing and validation of the instrument's optical performance.

    Main Results:

    • Successful design and construction of the modular spectrometer.
    • Demonstration of microsampling and cryosampling functionalities.
    • Preliminary results show effective optical characterization of superconducting materials.
    • Identification of instrument strengths for specific applications.

    Conclusions:

    • The developed infrared spectrometer is a capable tool for material analysis.
    • Microsampling and cryosampling enhance analytical flexibility.
    • The instrument shows promise for the optical characterization of superconductors.