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Related Experiment Video

Updated: Jun 8, 2026

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates
08:09

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates

Published on: May 9, 2014

Single-step bifunctional coating for selectively conjugable nanoparticles.

Valerio Voliani1, Stefano Luin, Fernanda Ricci

  • 1NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Piazza San Silvestro, 12, I-56127, Pisa, Italy. v.voliani@sns.it

Nanoscale
|October 12, 2010
PubMed
Summary
This summary is machine-generated.

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A novel peptide coating enables single-step functionalization of gold nanoparticles (NPs) with dual reactive groups. This method yields stable, biocompatible NPs for versatile biomolecule and fluorophore conjugation.

Area of Science:

  • Nanotechnology
  • Materials Science
  • Bioconjugation Chemistry

Background:

  • Gold nanoparticles (NPs) are widely used in biomedical applications.
  • Developing methods for stable and versatile NP functionalization is crucial.
  • Existing methods often involve multiple steps and may lack control over surface chemistry.

Purpose of the Study:

  • To report a single-step method for coating and bifunctionalizing gold nanoparticles.
  • To create stable, non-cytotoxic NPs with two distinct reactive surface groups.
  • To enable modular conjugation of biomolecules and fluorophores onto NPs.

Main Methods:

  • Utilizing a specifically designed peptide for NP coating and stabilization.
  • Employing N-cysteine for NP surface bonding and C-terminal lysine for functionalization.

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Last Updated: Jun 8, 2026

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates
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  • Characterizing functionalized NPs using electrophoresis, Scanning Electron Microscopy (SEM), and Surface-Enhanced Raman Spectroscopy (SERS).
  • Assessing biocompatibility and cellular uptake capabilities.

    • Main Results:

    • A stable, single-step coating and bifunctionalization of gold nanoparticles was achieved.
    • The peptide coating provides stability and presents both amine and carboxylic acid groups.
    • The functionalized NPs demonstrated non-cytotoxicity and efficient cellular uptake.
    • Successful conjugation of biomolecules and fluorophores was confirmed.

    Conclusions:

    • The developed peptide-based method offers a facile route to bifunctional gold nanoparticles.
    • These functionalized NPs are stable, biocompatible, and suitable for diverse conjugation strategies.
    • The method facilitates the development of advanced nanostructures for biological applications.