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Updated: Feb 9, 2026

A Purification and In Vitro Activity Assay for a pppGpp Synthetase from Clostridium difficile
Published on: November 3, 2018
Constant activity of glutamine synthetase after morphine administration versus proteomic results
Anna Bodzon-Kulakowska1, Piotr Suder, Anna Drabik
1Department of Biochemistry and Neurobiology, Faculty of Materials Science and Ceramics, University of Science and Technology, Mickiewicza 30 st, Krakow 30-059, Poland.
Abstract:
Glutamine synthetase is a key enzyme which has a regulatory role in the brain glutamate pool. According to previously published proteomic analysis, it was shown that the expression level of this enzyme is affected by morphine administration. In our study, we examined the activity of glutamine synthetase in various structures of rat brain (cortex, striatum, hippocampus and spinal cord) that are biochemically and functionally involved in drug addiction and antinociception caused by morphine. We were not able to observe any significant changes in the enzyme activity between morphine-treated and control samples despite previously reported changes in the expression levels of this enzyme. These findings stressed the fact that changes observed in the expression of particular proteins during proteomic studies may not be correlated with its activity.
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