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Updated: Jun 8, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Fast separation ultra-performance liquid chromatography for determination of pre-column derivative abamectin and
Hongcheng Liu1, Ying Zhang, Lianliang Liu
1Supervision & Testing Center for Farm Product Quality, Ministry of Agriculture, (Kunming), Institute of Quality Standard and Testing Technology, Yunnan Academy of Agriculture Science, 650223 Kunming, People's Republic of China.
Abstract:
A new residue method for quantification of abamectin and ivermectin in vegetable is described in the article. The derivative process is devised that acylating chemical is firstly performed by N-methylimidazole (MI) and trifluoroacetic anhydride (TFAA), then which reacted with hydroxyl function of abamerctin to make fluorescence. The influence of triethylamin (TEA) is examined. Separation is resolute by a short column of 1.7 μm size and operated at high pressure values (10.000 psi). The optimal chromatographic condition and the highest sensitivity are achieved by acetonitrile: water (95: 5), 0.4 mL/min, 0.2 μL injector. The detection limits of abamectin and ivermectin are 1 μg/kg respectively.
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