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Updated: Jun 8, 2026
Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
Published on: August 2, 2018
[Interleukin-10 inhibits the expression of interferon regulatory factor-1 with anoxia-reoxygenation in vitro]
Rong-Hua Chen1, Nan Liu, De-Shan Liu
1Department of Neurology, Union Hospital, Fujian Medical University, Fuzhou 350001, China. rong15@yahoo.cn
Aim:
to explore the effects of IL-10 on the expression of interferon regulatory factor-1 (IRF-1) after anoxia-reoxygenation in vitro.
Methods:
brain microvascular endothelial cells (BMEC) and neutrophil (PMN) were co-cultured exposed to eight hours of anoxia followed by two hours of reoxygenation to establish anoxia-reoxygenation model in vitro. The co-cultured BMEC and PMN were randomly divided into the following groups: normal group, anoxia-reoxygenation group, 1 microg/L IL-10 treatment group, 10 microg/L IL-10 treatment group, and 30 microg/L IL-10 treatment group. The expression of IRF-1 was detected by RT-PCR and Western blot method.
Results:
the expression of IRF-1 was significantly upregulated after anoxia-reoxygenation. IL-10 inhibited the expression of IRF-1 in a dose-dependent manner compared with anoxia-reoxygenation group, and the peak of inhibitive effects occurred at the dose of 30 μg/L.
Conclusion:
IL-10 can inhibit the expression of interferon regulatory factor-1 with anoxia-reoxygenation in vitro.
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