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[ZNF185 gene cloning and the localization in mouse testis]
Zhi-Fang Pan1, Wei-Guo Feng, Li-Mei Chen
1Department of Cell Biology, Weifang Medical University, Weifang 261053, China. sdaqfwg@126.com
Aim:
we Clone the ZNF185 gene and detect the position of ZNF185 in the mouse testis.
Methods:
extracted from mouse testis RNA, by RT-PCR, and then the obtained fragment was cloned and identified; extracted from mouse liver, testis and ovary proteins were Western blot analysis; preparation of frozen sections of mouse testes, immunofluorescence techniques analysis.
Results:
(1) ZNF185 gene cloning was correct. (2) Western blot showed that the most abundant in the testes ZNF185. (3) Immunofluorescence showed, ZNF185 located in Leydig cells and sperm, Leydig cells in the weak, and in round spermatids and mature sperm were highly expressed.
Conclusion:
the gene cloning of ZNF185 was successful and initially proved the position of ZNF185 in the mouse testis.
