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Published on: October 20, 2014
Neuronal activity and the expression of clathrin-assembly protein AP180
Fangbai Wu1, Mark P Mattson, Pamela J Yao
1Laboratory of Neurosciences, NIA/NIH, Baltimore, MD 21224, United States.
Abstract:
The clathrin-assembly protein AP180 is known to promote the assembly of clathrin-coated vesicles in the neuron. However, it is unknown whether the expression of AP180 is influenced by neuronal activity. In this study, we report that chronic depolarization results in a reduction of AP180 from hippocampal neurons, while acute depolarization causes a dispersed synaptic distribution of AP180. Activity-induced effects are observed only for AP180, but not for the structurally-related clathrin-assembly proteins CALM, epsin1, or HIP1. These findings suggest that AP180 levels and synaptic distribution are highly sensitive to neuronal activity.
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