Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Background and Environment Affect Phenotype

Background and Environment Affect Phenotype

Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...

Difference from Background: Limit of Detection

Difference from Background: Limit of Detection

The limit of detection (LOD) is the smallest amount of analyte that can be distinguished from the background noise. The LOD value corresponds to the concentration at which the analyte signal is three times larger than the standard deviation of the blank signal. Below this value, the analyte signal cannot be differentiated from the background noise. It is calculated by dividing the calibration slope by 3 times the standard deviation of the blank signals.
The LOD indicates the presence or absence...

Biostatistics: Overview

Biostatistics: Overview

Biostatistics plays a crucial role in understanding and analyzing data in healthcare and biology. Biostatisticians conduct experiments, gather evidence, and draw meaningful conclusions using statistical methods and techniques. Different variables form the foundation of biostatistical analysis, allowing researchers to understand and interpret data effectively. These variables are classified into different types, each serving a specific purpose in statistical analysis.
Discrete variables are...

Statistical Analysis: Overview

Statistical Analysis: Overview

When we take repeated measurements on the same or replicated samples, we will observe inconsistencies in the magnitude. These inconsistencies are called errors. To categorize and characterize these results and their errors, the researcher can use statistical analysis to determine the quality of the measurements and/or suitability of the methods.
One of the most commonly used statistical quantifiers is the mean, which is the ratio between the sum of the numerical values of all results and the...

Significance Testing: Overview

Significance Testing: Overview

Significance testing is a set of statistical methods used to test whether a claim about a parameter is valid. In analytical chemistry, significance testing is used primarily to determine whether the difference between two values comes from determinate or random errors. The effect of a particular change in the measurement protocol, analyst, or sample itself can cause a deviation from the expected result. In the case of a suspected deviation/outlier, we need to be able to confirm mathematically...

Mass Spectrometry: Overview

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Matrix Metalloproteinases in Periodontal and Peri-Implant Diseases: Contribution to Their Pathogenesis, Diagnosis, and Treatment.

Journal of periodontal research·2026

Same author

Diabetes, periodontal disease, and novel therapeutic approaches- host modulation therapy.

Frontiers in clinical diabetes and healthcare·2025

Same author

Host modulation therapy in periodontitis, diagnosis and treatment-status update.

Frontiers in dental medicine·2025

Same author

Effect of a chemically-modified-curcumin on dental resin biodegradation.

Frontiers in oral health·2025

Same author

A novel modified-curcumin 2.24 resolves inflammation by promoting M2 macrophage polarization.

Scientific reports·2023

Same author

Efficacy of a Novel Pleiotropic MMP-Inhibitor, CMC2.24, in a Long-Term Diabetes Rat Model with Severe Hyperglycemia-Induced Oral Bone Loss.

Journal of inflammation research·2023

Search research articles

Related Experiment Video

Updated: Jun 8, 2026

Mouse Dorsal Forebrain Explant Isolation

Mouse Dorsal Forebrain Explant Isolation

Published on: January 30, 2007

Introduction and background.

Lorne M Golub¹

¹Department of Oral Biology and Pathology, School of Dental Medicine, Stony Brook University, Stony Brook, New York 11794, USA. lorne.golub@stonybrook.edu

Pharmacological Research

|October 13, 2010

Summary

Tetracyclines (TCs) inhibit matrix metalloproteinases (MMPs) through non-antibiotic mechanisms, leading to approved drugs for periodontal and skin diseases. Further research explores chemically-modified TCs (CMTs) for cancer therapy.

Area of Science:

Pharmacology and Medicinal Chemistry: Focuses on the non-antimicrobial applications of tetracyclines.
Biochemistry: Investigates the inhibition of matrix metalloproteinases (MMPs) and cytokine activity.

Background:

Matrix metalloproteinases (MMPs) are key mediators of tissue destruction in various diseases, including bone loss.
Tetracyclines (TCs) were discovered to inhibit MMPs through mechanisms independent of their antibiotic properties.

Discussion:

Development of sub-antimicrobial-dose doxycycline led to approved treatments for periodontal and inflammatory skin diseases.
Chemically-modified tetracyclines (CMTs) show potential as anti-angiogenesis agents, with one candidate in cancer clinical trials.
Identification of the calcium and zinc binding site (carbons 11 and 12) as crucial for MMP inhibition by TCs.

Key Insights:

TCs offer therapeutic benefits beyond their antibiotic effects by targeting MMPs.

More Related Videos

Micro-scale Engineering for Cell Biology

Micro-scale Engineering for Cell Biology

Published on: October 1, 2007

Related Experiment Videos

Last Updated: Jun 8, 2026

Mouse Dorsal Forebrain Explant Isolation

Mouse Dorsal Forebrain Explant Isolation

Published on: January 30, 2007

Micro-scale Engineering for Cell Biology

Micro-scale Engineering for Cell Biology

Published on: October 1, 2007

Non-antimicrobial formulations of TCs have yielded successful clinical applications.

CMTs represent a novel class of therapeutic agents with anti-angiogenesis properties.

Outlook:

Balancing MMP inhibition is crucial, as basal levels are necessary for physiological functions.
Exploring TCs as inhibitors of intracellular MMP activity presents new therapeutic avenues.
Further research into CMTs for cancer and other MMP-related diseases is warranted.