Related Experiment Video
Updated: Jun 8, 2026

10:50
Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
Published on: March 9, 2010
Heme conjugated magnetic beads to isolate heme-binding proteins from complex mixtures
1Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX13QU, United Kingdom.
Protein Expression and Purification
|October 13, 2010
Abstract:
The cofactor heme (Fe-protoporphyrin IX) plays many important roles in biology. Identification of novel proteins for the transport, chaperoning and delivery of heme in cells is of widespread interest. Here, we describe the use of heme conjugated magnetic beads for the isolation of heme-binding proteins from complex protein mixtures. The reagent is straightforward to use, sensitive and specific.

