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Mycobacterial DNA Extraction using Bead Beating in Custom Buffer Followed by NGS Workflow
Published on: June 13, 2025
Nanobead-based interventions for the treatment and prevention of tuberculosis
Gareth Griffiths1, Bo Nyström, Suraj B Sable
1Department of Molecular Biosciences, University of Oslo, 0316 Oslo, Norway. g.w.griffiths@imbv.uio.no
Abstract:
Tuberculosis (TB), caused by Mycobacterium tuberculosis, is one of the most devastating bacterial diseases to affect humans. M. tuberculosis is a robust pathogen that has evolved the capacity to survive and grow inside macrophage phagosomes. A cocktail of antibiotics has long been successfully used against M. tuberculosis but is becoming less effective owing to the emergence of multidrug resistance. The only available preventive vaccine, using Mycobacterium bovis bacille Calmette-Guérin, is considered to be ineffective against adult pulmonary TB, the most prevalent form of the disease. Here, we review the potential use of biodegradable nanoparticle-based anti-TB drug delivery systems that have been shown to be more effective against M. tuberculosis in animal models than conventional antibiotic treatment regimens. This technology also has substantial potential for vaccination and other therapeutic strategies against TB and other infectious diseases.
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