Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthetic cannabinoids in human post-mortem samples - ADB-BUTINACA and metabolites in three fatalities.

Frontiers in toxicology·2026
Same author

Phase I Metabolism of Novel Phencyclidine Derivative 3-Cl-PCP: In Vitro Studies With Pooled Human Liver Microsomes and Investigation of a Post-Mortem Case.

Drug testing and analysis·2026
Same author

5-Methyl etodesnitazene human metabolism: LC-ESI<sup>±</sup>-HRMS/MS analysis (mono- and di-protonation) of human hepatocyte incubations and positive biospecimens.

Analytical and bioanalytical chemistry·2026
Same author

Acute toxicity of ADB-CHMINACA - a case series of patients with pronounced central nervous symptoms including the posterior reversible encephalopathic syndrome.

British journal of clinical pharmacology·2026
Same author

Virus-like and Virus Replicon Particles Targeting Multiple B-Cell Antigens Do Not Protect Against African Swine Fever Virus.

Vaccines·2026
Same author

Impact of non-genetic heterogeneity of BRAF-mutant colon cancer organoids on growth kinetics, drug sensitivity and Wnt dynamics.

International journal of cancer·2026

Related Experiment Video

Updated: Jun 8, 2026

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing
05:34

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

Published on: April 15, 2021

Continuous microfluidic DNA extraction using phase-transfer magnetophoresis.

Marc Karle1, Junichi Miwa, Gregor Czilwik

  • 1HSG-IMIT, Wilhelm-Schickard-Strasse 10, 78052, Villingen-Schwenningen, Germany. marc.karle@hsg-imit.de

Lab on a Chip
|October 13, 2010
PubMed
Summary

This study introduces a novel microfluidic chip for continuous DNA extraction using phase-transfer magnetophoresis. This platform enables rapid, automated biomolecule processing with high recovery rates.

More Related Videos

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers
11:21

Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers

Published on: August 30, 2024

Related Experiment Videos

Last Updated: Jun 8, 2026

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing
05:34

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

Published on: April 15, 2021

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers
11:21

Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers

Published on: August 30, 2024

Area of Science:

  • Biotechnology
  • Microfluidics
  • Molecular Biology

Background:

  • Continuous processing of biomolecules is crucial for automation.
  • Existing methods often rely on batch-wise processing, limiting throughput.
  • Microfluidic platforms offer miniaturization and precise control for biological assays.

Purpose of the Study:

  • To develop a novel microfluidic-chip based platform for continuous biomolecule processing.
  • To demonstrate continuous DNA extraction from bacterial cells using phase-transfer magnetophoresis.
  • To evaluate the efficiency and recovery rate of the developed platform.

Main Methods:

  • Utilized a microfluidic chip with co-flowing laminar streams.
  • Employed phase-transfer magnetophoresis for bead actuation via a rotating magnetic field.
  • Integrated cell lysis, DNA capture on magnetic beads, washing, and elution steps.
  • Formed flagella-like chains of magnetic beads for transport.

Main Results:

  • Achieved continuous DNA extraction from Escherichia coli with a turnover time of approximately 2 minutes.
  • Demonstrated high DNA recovery (147% on average) compared to batch methods.
  • Processed samples across a 7-log dilution series, showcasing robustness.
  • Operated at a sample flow rate of 0.75 µl/s and eluate flow rate of 0.35 µl/s.

Conclusions:

  • The developed microfluidic platform enables efficient, continuous DNA extraction.
  • Phase-transfer magnetophoresis is a viable technique for automated magnetic bead-based applications.
  • Potential applications include flow-through PCR, cell analysis, immunoassays, and environmental/epidemiological monitoring.